Collagen Type I antibody (AA 1051-1150)

Catalog No. ABIN2173309

Product Details anti-Collagen Type I Antibody

Target: Collagen Type I (COL1) (Collagen, Type I (COL1))

Binding Specificity: AA 1051-1150

Reactivity: Human, Rat, Mouse, Rabbit

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Collagen Type I antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunofluorescence (Cultured Cells) (IF (cc)), Immunocytochemistry (ICC), Flow Cytometry (FACS)

Cross-Reactivity: Human, Mouse, Rabbit, Rat

Predicted Reactivity: Dog,Cow,Sheep,Chicken

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human Collagen I

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
FCM 1:20-100
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200
ICC 1:100-500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

References for anti-Collagen Type I antibody (ABIN2173309)

Velázquez, Enos, Carson, Cranford, Bader, Sougiannis, Pritchett, Fan, Carson, Murphy: "miR155 deficiency aggravates high-fat diet-induced adipose tissue fibrosis in male mice." in: Physiological reports, Vol. 5, Issue 18, (2018) (PubMed).

Li, Xiong, Gong: "Lyn kinase enhanced hepatic fibrosis by modulating the activation of hepatic stellate cells." in: American journal of translational research, Vol. 9, Issue 6, pp. 2865-2877, (2017) (PubMed).

Miyauchi, Yasuchika, Fukumitsu, Ishii, Ogiso, Minami, Kojima, Yamaoka, Katayama, Kawai, Yoshitoshi-Uebayashi, Kita, Yasuda, Sasaki, Uemoto: "A novel three-dimensional culture system maintaining the physiological extracellular matrix of fibrotic model livers accelerates progression of hepatocellular carcinoma cells." in: Scientific reports, Vol. 7, Issue 1, pp. 9827, (2017) (PubMed).

Zhang, Dang, Li, Hao, Li, Qi: "Cardiac Contractility Modulation Attenuate Myocardial Fibrosis by Inhibiting TGF-β1/Smad3 Signaling Pathway in a Rabbit Model of Chronic Heart Failure." in: Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology, Vol. 39, Issue 1, pp. 294-302, (2017) (PubMed).

Zhou, Zhu, Zhang, Zhang, Zhou, Wang, He: "Imperatorin derivative OW1 inhibits the upregulation of TGF-β and MMP-2 in renovascular hypertension-induced cardiac remodeling." in: Experimental and therapeutic medicine, Vol. 11, Issue 5, pp. 1748-1754, (2016) (PubMed).

Zhu, Hu, Yu, Ren, Hu: "High Molecular Weight Hyaluronic Acid Inhibits Fibrosis of Endometrium." in: Medical science monitor : international medical journal of experimental and clinical research, Vol. 22, pp. 3438-3445, (2016) (PubMed).

Jiang, Chun, Wang, Du, Wang, Xiong: "Effect of transforming growth factor-β3 on the expression of Smad3 and Smad7 in tenocytes." in: Molecular medicine reports, Vol. 13, Issue 4, pp. 3567-73, (2016) (PubMed).

Liang, Li, Wang, Chen, Zhao, Sun, Zhuang, Shi, Shen, Zhang, Dai: "A collagen-binding EGFR antibody fragment targeting tumors with a collagen-rich extracellular matrix." in: Scientific reports, Vol. 6, pp. 18205, (2016) (PubMed).

Lu, Wang, Zhang, Zhou, Zuo, You, Gao, Liu, Tang: "A novel silk fibroin nanofibrous membrane for guided bone regeneration: a study in rat calvarial defects." in: American journal of translational research, Vol. 7, Issue 11, pp. 2244-53, (2016) (PubMed).

Hu, Zeng, Jiang, Hu, Meng, Zhu, Mao: "The expression of marker for endometrial stem cell and fibrosis was increased in intrauterine adhesious." in: International journal of clinical and experimental pathology, Vol. 8, Issue 2, pp. 1525-34, (2015) (PubMed).

Luo, Zhang, Wang, Hu, Song, Zhang: "The inhibitory effect of salmon calcitonin on intervertebral disc degeneration in an ovariectomized rat model." in: European spine journal : official publication of the European Spine Society, the European Spinal Deformity Society, and the European Section of the Cervical Spine Research Society, (2014) (PubMed).

Target Details for Collagen Type I

Target: Collagen Type I (COL1) (Collagen, Type I (COL1))

Alternative Name: Collagen I (COL1 Products)

Background:

Synonyms: OI1, OI2, OI3, OI4, EDSC, Collagen alpha-1(I) chain, Alpha-1 type I collagen, COL1A1l, CO1A1_HUMAN

Background: Type I collagen is a member of group I collagen (fibrillar forming collagen).

Gene ID: 1277

UniProt: P02452