TNF alpha antibody

Catalog No. ABIN217650

The Rat Monoclonal anti-TNF alpha antibody (Clone MP9-20A4) (ABIN217650) specifically detects TNF alpha in WB, IHC, ELISA, FACS and IHC (fro). The antibody is reactive with Human samples.

Quick Overview for TNF alpha antibody (ABIN217650)

Target: TNF alpha (Tumor Necrosis Factor alpha (TNF alpha))

Reactivity: Human

Host: Rat

Clonality: Monoclonal

Conjugate: This TNF alpha antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: MP9-20A4

Product Details anti-TNF alpha Antibody

Specificity: Recognizes human tumor Necrosis Factor - alpha (TNF alpha), also known as TNFSF2. This ~17kD cytokine is expressed by activated macrophages, monocytes, neutrophils, T cells and NK cells. Is reported to act as a blocking antibody.

Purification: Protein G purified

Immunogen: Recombinant TNF alpha

Type of Immunogen: Recombinant protein

Isotype: IgG1

Application Details

Application Notes: Approved: ELISA (5 - 10 μg/mL), Flo, IHC, IHC-Fr, WB

Usage: Flow Cytometry: Use 10 μL of the suggested working dilution to label 10^6 cells in 100 μL. Method sheets are available on request.

Not recommended for: IHC-P

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.4, 0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: 4°C or -20°C, Avoid freeze-thaw cycles.

Target Details for TNF alpha

Target: TNF alpha (Tumor Necrosis Factor alpha (TNF alpha))

Alternative Name: TNF alpha

Background: Name/Gene ID: TNF
Family: TNF

Synonyms: TNF, APC1 protein, Cachectin, DIF, TNF Alpha, Tumor necrosis factor-alpha, TNF-alpha, TNF-a, TNFA, Tumor necrosis factor, TNF, macrophage-derived, TNF, monocyte-derived, TNFSF2

Gene ID: 7124

Pathways: NF-kappaB Signaling, Apoptosis, Caspase Cascade in Apoptosis, TLR Signaling, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus, Inflammasome