c-MET antibody (pTyr1003) (Cy5)

Catalog No. ABIN2177600

The Rabbit Polyclonal anti-c-MET antibody has been validated for WB. It is suitable to detect c-MET in samples from Human, Mouse and Rat.

Quick Overview for c-MET antibody (pTyr1003) (Cy5) (ABIN2177600)

Target: c-MET (MET) (Met Proto-Oncogene (MET))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This c-MET antibody is conjugated to Cy5

Application: Western Blotting (WB)

Product Details anti-c-MET Antibody (Cy5)

Binding Specificity: pTyr1003

Cross-Reactivity: Human

Predicted Reactivity: Mouse,Rat,Dog,Cow,Sheep,Pig,Horse,Rabbit,Guinea Pig

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic phosphopeptide derived from human c-Met around the phosphorylation site of Tyr1003

Isotype: IgG

Application Details

Application Notes: IF(IHC-P) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for c-MET

Target: c-MET (MET) (Met Proto-Oncogene (MET))

Alternative Name: Met (C-Met)

Background:

Synonyms: Met c-Met Tyr1003, AUTS9, c met, cmet, D249, Hepatocyte growth factor receptor, Hepatocyte growth factor receptor Precursor, HGF, HGF receptor, HGF SF receptor, HGF/SF receptor, HGFR, MET, Met proto oncogene tyrosine kinase, Met proto-oncogene hepatocyte growth factor receptor, Met proto-oncogene, Met protooncogene, MET_HUMAN, Oncogene MET, Par4, Proto-oncogene c-Met, RCCP2, Renal cell carcinoma papillary 2 gene, Scatter factor receptor, SF receptor, Tyrosine-protein kinase Met.

Background: c-Met, a member of the tyrosine kinase superfamily, is the receptor for hepatocyte growth factor, also known as scatter factor (HGF/SF). The mature c-Met protein is a disulfide-linked heterodimer with Mr=190 kDa composed of a heavily glycosylated alpha subunit that is completely extracellular in localization, and a beta subunit comprising an extracellular ligand binding domain, a single transmembrane domain, and a cytoplasmic tyrosine kinase domain. Cells expressing c-Met include epithelial cells, endothelial cells, blood cells of various types, and glomerular mesenchymal cells.HGF/SF binding to c-Met stimulates receptor dimerization and the phosphorylation of numerous residues within the receptor?????s cytoplasmic domain. Signaling proteins that are phosphorylated and/or localized in response to c-Met phosphorylation include: Grb2, Shc, Cbl, Crk, cortactin, paxillin, GAB1, PI3K, FAK, Src, Ras, ERK1 and 2, JNK, PLC gamma, AKT, and STAT3. HGF/SF stimulation of c-Met expressing cells enhances proliferation, migration, morphogenesis, and protease synthesis, characteristics that are associated with invasive cell phenotype. Many types of cancer exhibit sustained c-Met stimulation, overexpression, or mutation, including carcinomas of the colon, breast, ovary, lung, liver, prostate, thyroid, kidney, as well as melanomas and sarcomas. In addition to cancer studies, other research areas in which c-Met is under investigation include organogenesis, organ regeneration, angiogenesis and surgical wound healing.

Gene ID: 4233

Pathways: RTK Signaling, Carbohydrate Homeostasis, Synaptic Membrane, Signaling of Hepatocyte Growth Factor Receptor