CDK9 antibody

Catalog No. ABIN218504

The Rabbit Polyclonal anti-CDK9 antibody is suitable to detect CDK9 in samples from Human, Mouse and Rat. It has been validated for WB, ELISA, IHC, IP, ICC and IHC (p).

Quick Overview for CDK9 antibody (ABIN218504)

Target: CDK9 (Cyclin-Dependent Kinase 9 (CDK9))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CDK9 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-CDK9 Antibody

Specificity: Multiple synthetic peptides corresponding to C-terminal and N-terminal domains of the protein coded by the human gene cdk9 (PITALRE).

Purification: Delipidated and defibrinated

Immunogen: Synthetic peptide from human CDK9.

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: ELISA (1:10000 - 1:50000), ICC, IHC, IHC-P (1:500), IP, WB (1:500 - 1:3000)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 1:500.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: 0.02 M potassium phosphate, 0.15 M sodium chloride,  pH 7.2, 0.01 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid cycles of freezing and thawing.

Storage: -20 °C

Storage Comment: Store vial at -20°C or below prior to opening. Dilute only prior to immediate use. Aliquot contents and freeze at -20°C or below. Avoid freeze-thaw cycles.

Target Details for CDK9

Target: CDK9 (Cyclin-Dependent Kinase 9 (CDK9))

Alternative Name: CDK9

Background: Name/Gene ID: CDK9
Subfamily: CDC2/CDK
Family: Protein Kinase

Synonyms: CDK9, C-2k, CDC2L4, CDC2-related kinase, Cell division protein kinase 9, Cyclin-dependent kinase 9, CDC2-related protein kinase, PITALRE, TAK, CTK1

Gene ID: 1025

UniProt: P50750

Pathways: Cell Division Cycle