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NF-kB p65 antibody (pSer529)

The Rabbit Polyclonal anti-NF-kB p65 antibody is suitable to detect NF-kB p65 in samples from Human, Monkey, Pig, Horse and Rabbit. It has been validated for WB, IHC, ELISA, IP and IHC (p).
Catalog No. ABIN218582
$633.60
Plus shipping costs $50.00
50 μL
Shipping to: United States
Delivery in 11 to 15 Business Days

Quick Overview for NF-kB p65 antibody (pSer529) (ABIN218582)

Target

See all NF-kB p65 (NFkBP65) Antibodies
NF-kB p65 (NFkBP65) (Nuclear Factor-kB p65 (NFkBP65))

Reactivity

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Human, Monkey, Pig, Horse, Rabbit

Host

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Rabbit

Clonality

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Polyclonal

Conjugate

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This NF-kB p65 antibody is un-conjugated

Application

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Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
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    pSer529

    Specificity

    NFkB p65 (Rel A) peptide corresponding to a region near phospho Serine 529 of the human protein conjugated to Keyhole Limpet Hemocyanin (KLH). Sequence information: PNGLLpSGDEDFC.

    Purification

    Delipidated and defibrinated

    Immunogen



    Type of Immunogen: Native protein
  • Application Notes

    Approved: ELISA, IHC, IHC-P (1:500), IP, WB

    Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 1:500.

    Comment

    Target Species of Antibody: Human

    Restrictions

    For Research Use only
  • Format

    Liquid

    Concentration

    Lot specific

    Buffer

    Serum, 0.1 % sodium azide.

    Preservative

    Sodium azide

    Precaution of Use

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Handling Advice

    Avoid repeated freezing and thawing.

    Storage

    4 °C,-20 °C

    Storage Comment

    4°C or -20°C, Avoid freeze-thaw cycles.
  • Target

    NF-kB p65 (NFkBP65) (Nuclear Factor-kB p65 (NFkBP65))

    Alternative Name

    RELA / NFKB p65

    Background

    Name/Gene ID: RELA

    Synonyms: RELA, NF-kappa-B p65delta3, NFKB3, p65, Transcription factor p65

    Gene ID

    5970

    UniProt

    Q04206

    Pathways

    NF-kappaB Signaling, RTK Signaling, TCR Signaling, TLR Signaling, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Hepatitis C, Toll-Like Receptors Cascades, S100 Proteins
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