ASIC3 antibody (AA 285-304)

Catalog No. ABIN219004

This Guinea Pig Polyclonal antibody specifically detects ASIC3 in IHC and ICC. It exhibits reactivity toward Rat.

Quick Overview for ASIC3 antibody (AA 285-304) (ABIN219004)

Target: ASIC3 (ACCN3) (Amiloride-Sensitive Cation Channel 3 (ACCN3))

Reactivity: Rat

Host: Guinea Pig

Clonality: Polyclonal

Conjugate: This ASIC3 antibody is un-conjugated

Application: Immunohistochemistry (IHC), Immunocytochemistry (ICC)

Product Details anti-ASIC3 Antibody

Binding Specificity: AA 285-304

Specificity: Recognizes rat ASIC3.

Predicted Reactivity: Percent identity by BLAST analysis: Rat (100%) Mouse (90%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide (C-TASLDPDDFDPEPSDPLGSP) residues 285-304 from the extracellular domain of rat ASIC3. Percent identity by BLAST analysis: Rat (100%), Mouse (90%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: ICC (1:50), IHC (1:50)

Usage: Suitable for use in Immunohistochemistry and Immunocytochemistry. Immunohistochemistry (perfusion fixed tissue): 1:50. Perfusion: 1) calcium-free Tyrode's solution, 2) paraformaldehyde-picric acid fixative, and 3) 10 % sucrose in PBS as cryo-protectant. Desired tissues were dissected and stored overnight in 10 % sucrose, PBS. Immunocytochemistry: 1:50. ASIC3 transfected cells and primary neuronal cell cultures.

Comment:

Target Species of Antibody: Rat

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.4, 1 % BSA, 0.05 % sodium azide, 40 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for ASIC3

Target: ASIC3 (ACCN3) (Amiloride-Sensitive Cation Channel 3 (ACCN3))

Alternative Name: ACCN3 / ASIC3

Background: Name/Gene ID: ASIC3
Subfamily: Sodium channel - amiloride-sensitive
Family: Ion Channel

Synonyms: ASIC3, Acid-sensing ion channel 3, Acid-sensing ion channel, ACCN3, HASIC3, HTNaC1, Modulatory subunit of ASIC2a, SLNAC1, DRASIC, Testis sodium channel 1, TNaC1

Gene ID: 9311

UniProt: Q9UHC3