ErbB2/Her2 antibody (Cytoplasmic Domain)

Catalog No. ABIN219136

This Mouse Monoclonal antibody specifically detects ErbB2/Her2 in WB, IHC and IP. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for ErbB2/Her2 antibody (Cytoplasmic Domain) (ABIN219136)

Target: ErbB2/Her2 (Receptor tyrosine-protein kinase erbB-2 (ErbB2/Her2))

Reactivity: Human, Mouse, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This ErbB2/Her2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP)

Product Details anti-ErbB2/Her2 Antibody

Binding Specificity: Cytoplasmic Domain

Specificity: Recognizes human c-erbB-2 (HER-2, neu). Species cross-reactivity: Mouse and rat.

Purification: Protein G purified

Immunogen: Cytoplasmic domain of recombinant human c-erbB-2/HER-2 oncoprotein. MW of Antigen: 185kD. Epitope: Cytoplasmic domain. Cellular Localization: Cell membrane.

Isotype: IgG1

Application Details

Application Notes: Approved: IHC (2 - 4 μg/mL), IP, WB (1 - 2 μg/mL)

Usage: Suitable for use in Immunoprecipitation, Western Blot and Immunohistochemistry. Immunoprecipitation (Native and Denatured) (Use Protein G): 2 μg/mg protein lysate. Western Blot: 1-2 μg/mL for 2hr at RT. Immunohistochemistry: 2-4 μg/mL for 30 min at RT. Note: Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 min. Positive control: SKBR-3 or T47D cells, or breast carcinoma.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.4, 0.2 % BSA, 0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for ErbB2/Her2

Target: ErbB2/Her2 (Receptor tyrosine-protein kinase erbB-2 (ErbB2/Her2))

Alternative Name: ERBB2 / HER2

Background: Name/Gene ID: ERBB2
Subfamily: EGF Receptor
Family: Protein Kinase

Synonyms: ERBB2, C-erb B2/neu protein, CD340, HER2, Herstatin, MLN 19, HER-2, Neu, NGL, Proto-oncogene c-ErbB-2, Proto-oncogene Neu, CD340 antigen, MLN19, p185erbB2, TKR1

Gene ID: 2064

UniProt: P04626

Pathways: RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Skeletal Muscle Fiber Development