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LBP antibody (Lipopolysaccharide Binding Protein) Primary Antibody

LBP Reactivity: Mouse BR, Func, IA, WB Host: Rat Monoclonal M330-19 unconjugated
Pubmed (2)
Catalog No. ABIN2191765
$650.44
Plus shipping costs $45.00
100 μg
local_shipping Shipping to: United States
Delivery in 6 to 8 Business Days
  • Target
    LBP
    Reactivity
    • 40
    • 19
    • 13
    • 8
    • 7
    • 6
    • 4
    • 3
    • 3
    • 2
    • 2
    Mouse
    Host
    • 41
    • 18
    • 3
    • 2
    Rat
    Clonality
    • 43
    • 21
    Monoclonal
    Conjugate
    • 54
    • 6
    • 2
    • 1
    • 1
    This LBP antibody is un-conjugated
    Application
    • 45
    • 29
    • 19
    • 13
    • 9
    • 9
    • 5
    • 4
    • 4
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    Blocking Reagent (BR), Functional Studies (Func), Immunoassay (IA), Western Blotting (WB)
    Sterility
    0.2 μm filtered
    Clone
    M330-19
  • Application Notes
    For Western blotting dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:10. For neutralization of biological activity dilutions have to be made according to the amounts LBP to be inactivated. Before use in biological assays, the product must be filter sterilized and depending on the concentration to be used dialyzed against culture medium to remove the sodium azide added. Please inquire for availability of azide free solutions.
    Restrictions
    For Research Use only
  • Buffer
    PBS, containing 0.02 % sodium azide and 0.1 % bovine serum albumin.
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    4 °C
    Storage Comment
    Product should be stored at 4 °C. Under recommended storage conditions, product is stable for one year.
    Expiry Date
    12 months
  • Le Roy, Di Padova, Adachi, Glauser, Calandra, Heumann: "Critical role of lipopolysaccharide-binding protein and CD14 in immune responses against gram-negative bacteria." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 167, Issue 5, pp. 2759-65, 2001 (PubMed).

    Le Roy, Di Padova, Tees, Lengacher, Landmann, Glauser, Calandra, Heumann et al.: "Monoclonal antibodies to murine lipopolysaccharide (LPS)-binding protein (LBP) protect mice from lethal endotoxemia by blocking either the binding of LPS to LBP or the presentation of LPS/LBP ..." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 162, Issue 12, pp. 7454-60, 1999 (PubMed).

  • Target
    LBP
    Alternative Name
    Lipopolysaccharide Binding Protein (LBP Antibody Abstract)
    Synonyms
    BPIFD2, Bpifd2, Ly88, LPSBP, LBP, lipopolysaccharide binding protein, lipopolysaccharide-binding protein, LBP, Lbp, LOC100472839
    Background
    The monoclonal antibody M330-19 reacts highly specific with mouse natural and recombinant LBP. The antibody is a type I antibody blocking the LPS binding to LBP. LPS binding protein (LBP) is an approximately 60 kDa acute phase protein that is produced by hepatocytes. This protein strongly binds to LPS and has been shown to play an important role in the handling of LPS by the host. A number of functions of LBP have been reported. First, LBP transfers LPS to the LPS receptor CD14 on mononuclear phagocytes, leading to an 100-1,000-fold increased sensitivity of the cells to LPS. Furthermore, LBP can enhance the response of CD14 negative cells by acceleration of LPS binding to soluble CD14, a complex that stimulates these cells. Next, LBP transfers LPS into High Density Lipoprotein (HDL), which effectively neutralizes its biological potency. LBP was demonstrated to protect mice from septic shock caused by LPS or gram negative bacteria.
    Pathways
    TLR Signaling, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Positive Regulation of Immune Effector Process, Toll-Like Receptors Cascades, Monocarboxylic Acid Catabolic Process
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