Complement 9 antibody (FITC)

Catalog No. ABIN2191991

Product Details

Target: Complement 9

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: FITC

Application: Western Blotting (WB), Immunoassay (IA), Immunohistochemistry (Frozen Sections) (IHC (fro)), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Sterility: 0.2 μm filtered

Clone: X197

Application Details

Application Notes: For immunohistology, flow cytometry and Western blotting dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:10.

Restrictions: For Research Use only

Handling

Buffer: PBS, containing 1 % bovine serum albumin and 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Product should be stored at 4 °C. Under recommended storage conditions, product is stable for one year.

Expiry Date: 12 months

References

Hatanaka, Seya, Yoden, Fukamoto, Semba, Inai: "Analysis of C5b-8 binding sites in the C9 molecule using monoclonal antibodies: participation of two separate epitopes of C9 in C5b-8 binding." in: Molecular immunology, Vol. 29, Issue 7-8, pp. 911-6, (1992) (PubMed).

Target Details

Target: Complement 9

Background: Human C9 is a soluble glycoprotein of 61 kD. It is the last component in the assembly of the membrane attack complex (MAC). When the complement is activated on target membranes, multiple copies of C9 bind to the C5b-8 complex and assemble the barrel-shaped pore which causes cell lysis. The conformation of C9 changes from globular to a tubular form. The binding of C9 to C5b-8 plays a key role in the function of C9.