Coli Elongation Factor Tu antibody

Catalog No. ABIN2192155

Product Details

Target: Coli Elongation Factor Tu

Reactivity: E. coli

Host: Mouse

Clonality: Monoclonal

Application: Immunoassay (IA), Immunofluorescence (IF), Western Blotting (WB)

Cross-Reactivity (Details): Cross reactivity: Bacteroides fragilis : Yes, Streptococcus oralis : Yes, Bacillus subtilis : Yes, Mycobacterium tuberculosis Weak Pseudomonas aeruginosa : Yes, Burkholderia cepacia : Yes, Deinococcus sp. : Yes

Sterility: 0.2 μm filtered

Clone: 9E2

Isotype: IgG2a

Application Details

Application Notes: For Western blotting, dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50.

Restrictions: For Research Use only

Handling

Buffer: PBS, containing 0.1 % bovine serum albumin and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Product should be stored at 4 °C. Under recommended storage conditions, product is stable for one year.

Expiry Date: 12 months

References

Kunert, Losse, Gruszin, Hühn, Kaendler, Mikkat, Volke, Hoffmann, Jokiranta, Seeberger, Moellmann, Hellwage, Zipfel: "Immune evasion of the human pathogen Pseudomonas aeruginosa: elongation factor Tuf is a factor H and plasminogen binding protein." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 179, Issue 5, pp. 2979-88, (2007) (PubMed).

Baensch, Frank, Köhl: "Conservation of the amino-terminal epitope of elongation factor Tu in eubacteria and Archaea." in: Microbiology (Reading, England), Vol. 144 ( Pt 8), pp. 2241-6, (1998) (PubMed).

Weber, Lottspeich, Köhl: "An epitope of elongation factor Tu is widely distributed within the bacterial and archaeal domains." in: Journal of bacteriology, Vol. 177, Issue 1, pp. 11-9, (1995) (PubMed).

Target Details

Target: Coli Elongation Factor Tu

Background: The monoclonal antibody 900 recognizes elongation factor Tu (EF-Tu), a 43 kDa multifunctional protein present in Escherichia coli. EF-Tu is one of the most abundant proteins present in prokaryotes, representing about 5 % of the total cellular protein of E. coli. During protein biosynthesis, the elongation process, EF-Tu catalyzes the binding of each aminoacyl-tRNA to the ribosome. It also interacts with several macromolecules and guanine nucleotides, including EF-Ts, GDP, GTP, and some ribosomal proteins. Monoclonal antibody 900 recognizes EF-Tu in organisms belonging to bacterial and archaeal domains, yet no organisms from the eukaryotic domain. The panbacterial distribution of EF-Tu, which is present in large amounts in every prokaryotic cell, renders this protein a good candidate for diagnostic purposes. The highly conserved epitope recognized by monoclonal antibody 900 is located at the very end of the N-terminus of the EF-Tu molecule (SKEKFE). Aliases EF-TU, Elongation factor TUF, Elongation factor Tu Immunogen E. coli K-12 C600 Rif ( pKT146) bacteria