Caspase 9 antibody (Asp353)

Catalog No. ABIN219281

The Rabbit Polyclonal anti-Caspase 9 antibody is suitable to detect Caspase 9 in samples from Mouse. It has been validated for WB, IF and ICC.

Quick Overview for Caspase 9 antibody (Asp353) (ABIN219281)

Target: Caspase 9 (CASP9) (Caspase 9, Apoptosis-Related Cysteine Peptidase (CASP9))

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Caspase 9 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC)

Product Details anti-Caspase 9 Antibody

Binding Specificity: Asp353

Specificity: Detects endogenous levels of the 37kD subunit of mouse Caspase-9 only after cleavage at aspartic acid 353. Does not cross-react with full length caspase-9 or with other caspases at endogenous levels.

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide (KLH-coupled) corresponding to residues surroundingAsp353 of mouse caspase-9.

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: ICC, IF (1:100), WB (1:1000)

Usage: Suitable for use in Western Blot, Immunocytochemistry. Western Blot: 1:1000. Immunocytochemistry: (IF) 1:100.

Comment:

Target Species of Antibody: Mouse

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: 10 mM sodium HEPES,  pH 7.5, 150 mM sodium chloride, 100 μg/mL BSA, 50 % glycerol.

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for Caspase 9

Target: Caspase 9 (CASP9) (Caspase 9, Apoptosis-Related Cysteine Peptidase (CASP9))

Alternative Name: CASP9 / Caspase 9

Background: Name/Gene ID: CASP9
Subfamily: Cysteine C14
Family: Protease

Synonyms: CASP9, APAF3, CASPASE-9c, ICE-LAP6, MCH6, ICE-like apoptotic protease 6, APAF-3, Apoptotic protease Mch-6, CASP-9, Caspase 9, Caspase-9, PPP1R56

Gene ID: 842

UniProt: P55211

Pathways: MAPK Signaling, RTK Signaling, Apoptosis, Caspase Cascade in Apoptosis, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Positive Regulation of Endopeptidase Activity