FOXO1 antibody (pSer256)

Catalog No. ABIN220191

This anti-FOXO1 antibody is a Rabbit Polyclonal antibody detecting FOXO1 in WB. Suitable for Human, Mouse and Rat.

Quick Overview for FOXO1 antibody (pSer256) (ABIN220191)

Target: FOXO1 (Forkhead Box O1 (FOXO1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This FOXO1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-FOXO1 Antibody

Binding Specificity: pSer256

Specificity: Detects endogenous levels of total levels of FKHR only when phosphorylated at Ser256. Cross-reacts with phosphorylated AFX. Species cross-reactivity: Human, mouse, rat.

Purification: Protein A purified

Immunogen: Synthetic phosphopeptide (KLH coupled) corresponding to residues around Ser256 of human FKHR.

Type of Immunogen: Synthetic phospho-peptide

Isotype: IgG

Application Details

Application Notes: Approved: WB (1:1000)

Usage: Suitable for use in Western Blot. Western Blot: 1:1000. Incubate membrane with diluted antibody in 5 % BSA, 1X TBS, 0.1 % Tween 20 at 4?C with gentle shaking overnight.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: 10 mM HEPES,  pH 7.5, 150 mM sodium chloride, 0.1 mg/mL BSA, 50 % glycerol. No preservative added.

Preservative: Without preservative

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for FOXO1

Target: FOXO1 (Forkhead Box O1 (FOXO1))

Alternative Name: FOXO1 / FKHR

Background: Name/Gene ID: FOXO1
Family: Transcription factor

Synonyms: FOXO1, FKHR, Forkhead in rhabdomyosarcoma, FOXO1A, FKH1, Forkhead box protein O1, Forkhead box protein O1A, Forkhead box O1, Forkhead box o1a, Forkhead domain protein fkhr

Gene ID: 2308

UniProt: Q12778

Pathways: PI3K-Akt Signaling, Cell Division Cycle, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Carbohydrate Homeostasis, Chromatin Binding, Regulation of Carbohydrate Metabolic Process, CXCR4-mediated Signaling Events, BCR Signaling