ERK1 antibody (pThr202, Tyr204)

Catalog No. ABIN220486

This Rabbit Polyclonal antibody specifically detects ERK1 in WB, ELISA, IF, IP, ICC and FACS. It exhibits reactivity toward Human, Mouse, Rat, Chicken and Hamster.

Quick Overview for ERK1 antibody (pThr202, Tyr204) (ABIN220486)

Target: ERK1 (MAPK3) (Mitogen-Activated Protein Kinase 3 (MAPK3))

Reactivity: Human, Mouse, Rat, Chicken, Hamster

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ERK1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunoprecipitation (IP), Immunocytochemistry (ICC), Flow Cytometry (FACS)

Product Details anti-ERK1 Antibody

Binding Specificity: pThr202, Tyr204

Specificity: Detects endogenous levels of human p42 and p44 MAP kinase (Erk1 and Erk2) only when phosphorylated either individually or dually at Thr202 and Thr204 of Erk1 (Thr185 an Tyr187 of Erk2). Does not cross-react with the corresponding phosphorylated residues of either JNK/SAPK or p38 MAP kinase, or non-phosphorylated Erk1/2. Species cross-reactivity: chicken, hamster, mouse, rat, zebrafish. bovine and monkey.

Purification: Immunoaffinity purified

Immunogen: Synthetic phosphopeptide corresponding to residues around Thr202/Tyr204 of human p44 MAP kinase (KLH).

Type of Immunogen: Synthetic phospho-peptide

Isotype: IgG

Application Details

Application Notes: Approved: ELISA, Flo (1:100), ICC, IF, IP (1:50), WB (1:1000)

Usage: Suitable for use in Flow Cytometry, ELISA, Western Blot, Immunoprecipitation, Immunofluorescence and Immunocytochemistry. Western Blot: 1:1000. Incubate membrane with diluted antibody in TBS, 5 % BSA, 0.1 % Tween-20 at 4?C with gentle shaking, overnight. Immunoprecipitation: 1:50. Immunofluorescence (IF-IC): 1:1000. Flow Cytometry: 1:100.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: 10 mM HEPES,  pH 7.5, 150 mM sodium chloride, 0.1 mg/mL BSA, 50 % glycerol. No preservative added.

Preservative: Without preservative

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for ERK1

Target: ERK1 (MAPK3) (Mitogen-Activated Protein Kinase 3 (MAPK3))

Alternative Name: MAPK3 / ERK1

Background: Name/Gene ID: MAPK3
Subfamily: MAPK
Family: Protein Kinase

Synonyms: MAPK3, ERK-1, ERT2, HUMKER1A, HS44KDAP, MAP kinase 1, MAP kinase isoform p44, MAPK 1, MAPK 3, p44, p44MAPK, p44-MAPK, p44ERK1, Insulin-stimulated MAP2 kinase, MAP kinase 3, p44-ERK1, ERK1, PRKM3

Gene ID: 5595

UniProt: P27361

Pathways: MAPK Signaling, RTK Signaling, Interferon-gamma Pathway, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway, Response to Growth Hormone Stimulus, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Hepatitis C, Protein targeting to Nucleus, Toll-Like Receptors Cascades, Signaling Events mediated by VEGFR1 and VEGFR2, Signaling of Hepatocyte Growth Factor Receptor, VEGFR1 Specific Signals, S100 Proteins