MSK1 antibody (pThr581)

Catalog No. ABIN220562

The Rabbit Polyclonal anti-MSK1 antibody is suitable to detect MSK1 in samples from Human and Mouse. It has been validated for WB, IHC and IHC (p).

Quick Overview for MSK1 antibody (pThr581) (ABIN220562)

Target: MSK1 (RPS6KA5) (Ribosomal Protein S6 Kinase, 90kDa, Polypeptide 5 (RPS6KA5))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MSK1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-MSK1 Antibody

Binding Specificity: pThr581

Specificity: Detects endogenous levels of human MSK1 only when activated by phosphorylation at Thr581. Does not cross-react with MSK1 phosphorylated at other sites, nor does it detect phosphorylated RSK1, RSK2 or RSK3. Species cross-reactivity: Mouse.

Purification: Protein A purified

Immunogen: Synthetic phosphopeptide (KLH coupled) corresponding to residues surrounding Thr581 of human MSK1.

Type of Immunogen: Synthetic phospho-peptide

Isotype: IgG

Application Details

Application Notes: Approved: IHC, IHC-P, WB (1:1000)

Usage: Western Blot: 1:1000. Immunohistochemistry (Paraffin): 1:50.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: 10 mM HEPES,  pH 7.5, 150 mM sodium chloride, 0.1 mg/mL BSA, 50 % glycerol. No preservative added.

Preservative: Without preservative

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for MSK1

Target: MSK1 (RPS6KA5) (Ribosomal Protein S6 Kinase, 90kDa, Polypeptide 5 (RPS6KA5))

Alternative Name: RPS6KA5 / MSK1

Background: Name/Gene ID: RPS6KA5
Subfamily: MSK
Family: Protein Kinase

Synonyms: RPS6KA5, MSPK1, RLPK, MSK1, RSK-like protein kinase, RSKL, S6K-alpha-5

Gene ID: 9252

UniProt: O75582

Pathways: MAPK Signaling, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Toll-Like Receptors Cascades