RNF7 antibody (C-Term)

Catalog No. ABIN221169

This anti-RNF7 antibody is a Rabbit Polyclonal antibody detecting RNF7 in ELISA, WB and IP. Suitable for Human.

Quick Overview for RNF7 antibody (C-Term) (ABIN221169)

Target: RNF7 (Ring Finger Protein 7 (RNF7))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RNF7 antibody is un-conjugated

Application: ELISA, Western Blotting (WB), Immunoprecipitation (IP)

Product Details anti-RNF7 Antibody

Binding Specificity: C-Term

Specificity: Amino acids 102-113 of Human ROC2 (C-terminal) coupled to KLH.

Purification: Delipidated and defibrinated

Immunogen: Synthetic peptide from human RNF7.

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: ELISA (1:2000 - 1:10000), IP, WB (1:500 - 1:1000)

Usage: This antibody reacts with human ROC2 by western blot and immunoprecipitation. The antibody immunoprecipitates in vitro translated protein and protein from overexpressing cell lysates (using HeLa and NIH-3T3, and others). Coimmunoprecipitation of related proteins does occur. A 12.6 kD band corresponding to human ROC2 is detected. Most cell lines expressing ROC2 can be used as a positive control. Researchers should determine optimal titers for other applications.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Sterile filtered antiserum, 0.01 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature.

Storage: -20 °C

Storage Comment: Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid freeze-thaw cycles. Centrifuge product if not completely clear after standing at room temperature.

Target Details for RNF7

Target: RNF7 (Ring Finger Protein 7 (RNF7))

Alternative Name: RNF7

Background: Name/Gene ID: RNF7

Synonyms: RNF7, CKII beta-binding protein 1, CKBBP1, RING-box protein 2, Rbx2, Regulator of cullins 2, Ring finger protein 7, ROC2, Zinc RING finger protein SAG

Gene ID: 9616

UniProt: Q9UBF6

Pathways: Positive Regulation of Endopeptidase Activity