Aurora A antibody (N-Term)

Catalog No. ABIN222101

The Rabbit Polyclonal anti-Aurora A antibody has been validated for WB and ELISA. It is suitable to detect Aurora A in samples from Human, Mouse and Monkey.

Quick Overview for Aurora A antibody (N-Term) (ABIN222101)

Target: Aurora A (AURKA) (Aurora Kinase A (AURKA))

Reactivity: Human, Mouse, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Aurora A antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-Aurora A Antibody

Binding Specificity: N-Term

Specificity: Detects endogenous levels of Aurora 2/AIK proteins. This antibody may cross-react with AIK3. Species cross-reactivity: Human, monkey and mouse.

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide (KLH coupled) corresponding to the N-terminus of human Aurora 2/AIK.

Type of Immunogen: Synthetic peptide - KLH conjugated

Isotype: IgG

Application Details

Application Notes: Approved: ELISA, WB (1:1000)

Usage: Suitable for use in ELISA, and Western Blot. Western Blot: 1:1000. Incubate membrane with diluted antibody in 5 % BSA, 1X TBS, 0.1 % Tween-20 at 4?C with gentle shaking, overnight.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: 10 mM HEPES,  pH 7.5, 150 mM sodium chloride, 0.1 mg/mL BSA, 50 % glycerol. No preservative added.

Preservative: Without preservative

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for Aurora A

Target: Aurora A (AURKA) (Aurora Kinase A (AURKA))

Alternative Name: AURKA / Aurora-A

Background: Name/Gene ID: AURKA
Subfamily: Aurora/IPL1-like
Family: Protein Kinase

Synonyms: AURKA, AIK, AIRK1, ARK1, Aurora-A kinase, Aurora kinase A, Aurora/IPL1-like kinase, AYK1, AURA, AURORA2, Breast tumor-amplified kinase, Breast-tumor-amplified kinase, BTAK, HARK1, IPL1-related kinase, Serine/threonine kinase 6, STK7, STK6, PPP1R47, ARK-1, Aurora 2, Aurora-A, Aurora-related kinase 1, Aurora/IPL1-related kinase 1, IAK1, Serine/threonine kinase 15, STK15

Gene ID: 6790

UniProt: O14965

Pathways: Cell Division Cycle, Asymmetric Protein Localization