RPS6 antibody (pSer235, Ser236)

Catalog No. ABIN222230

The Rabbit Monoclonal anti-RPS6 antibody has been validated for WB, IHC, IF, IHC (p), IP, ICC and IHC (fro). It is suitable to detect RPS6 in samples from Human and Mouse.

Quick Overview for RPS6 antibody (pSer235, Ser236) (ABIN222230)

Target: RPS6 (Ribosomal Protein S6 (RPS6))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Monoclonal

Conjugate: This RPS6 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-RPS6 Antibody

Binding Specificity: pSer235, Ser236

Specificity: Detects endogenous levels of human ribosomal protein S6 only when phosphorylated at serines 235 and 236. Species cross-reactivity: mouse and rat.

Purification: Purified

Immunogen: Synthetic phospho-peptide (KLH-coupled) corresponding to residues surrounding Ser235 and Ser236 of human ribosomal protein S6.

Type of Immunogen: Synthetic phospho-peptide

Isotype: IgG

Application Details

Application Notes: Approved: ICC (1:100), IF, IHC, IHC-Fr, IHC-P, IP, WB (1:1000)

Usage: Suitable for use in Western Blot, Immunohistochemistry, and Immunoprecipitation. Western Blot: 1:1000. Good for 10 mini blots. Immunohistochemistry (paraffin): 1:75. Immunohistochemistry (frozen): 1:75. Immunofluorescence/Immunocytochemistry: 1:100. Fixative: 10 % neutral buffered formalin.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: 10 mM sodium HEPES,  pH 7.5, 150 mM sodium chloride, 0.1 mg/mL BSA, 50 % glycerol.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for RPS6

Target: RPS6 (Ribosomal Protein S6 (RPS6))

Alternative Name: RPS6 / S6

Background: Name/Gene ID: RPS6

Synonyms: RPS6, 40S ribosomal protein S6, S6, Ribosomal protein S6, Phosphoprotein NP33

Gene ID: 6194

UniProt: P62753

Pathways: Carbohydrate Homeostasis, Ribonucleoprotein Complex Subunit Organization, Ribosome Assembly