RPS6 antibody (pSer235, Ser236)

Catalog No. ABIN222236

The Rabbit Polyclonal anti-RPS6 antibody has been validated for WB, ELISA, IHC, IF, IHC (p), FACS, IP and ICC. It is suitable to detect RPS6 in samples from Human, Mouse and Rat.

Quick Overview for RPS6 antibody (pSer235, Ser236) (ABIN222236)

Target: RPS6 (Ribosomal Protein S6 (RPS6))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RPS6 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Flow Cytometry (FACS), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Product Details anti-RPS6 Antibody

Binding Specificity: pSer235, Ser236

Specificity: Detects endogenous levels of human ribosomal protein S6 only when phosphorylated at Ser235/236 (Mr 32kD). Does not detect ribosomal protein S6 phosphorylated at other sites. Species cross-reactivity: Mouse, rat and zebrafish. Species sequence homology: chicken and Xenopus 100 % .

Purification: Protein A purified

Immunogen: Synthetic phosphopeptide corresponding to residues surrounding Ser235 and Ser236 of human ribosomal protein S6 (KLH coupled).

Type of Immunogen: Synthetic phospho-peptide

Isotype: IgG

Application Details

Application Notes: Approved: ELISA, Flo (1:50), ICC, IF, IHC, IHC-P, IP (1:100), WB (1:1000)

Usage: Suitable for use in ELISA, Western Blot, Immunoprecipitation, Immunohistochemistry, Immunocytochemistry and Flow Cytometry. Western Blot: 1:1000, incubate membrane with diluted antibody in 5 % BSA, 1X TBS, 0.1 % Tween-20 at 4?C with gentle shaking, overnight. Immunoprecipitation: 1:100. Immunohistochemistry (Paraffin): 1:50. Immunocytochemistry (for IF): 1:50. Flow Cytometry: 1:50.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: 10 mM HEPES,  pH 7.5, 150 mM sodium chloride, 0.1 mg/mL BSA, 50 % glycerol. No preservative added.

Preservative: Without preservative

Target Details for RPS6

Target: RPS6 (Ribosomal Protein S6 (RPS6))

Alternative Name: RPS6 / S6

Background: Name/Gene ID: RPS6

Synonyms: RPS6, 40S ribosomal protein S6, S6, Ribosomal protein S6, Phosphoprotein NP33

Gene ID: 6194

UniProt: P62753

Pathways: Carbohydrate Homeostasis, Ribonucleoprotein Complex Subunit Organization, Ribosome Assembly