BAX antibody (N-Term)
Quick Overview for BAX antibody (N-Term) (ABIN222445)
Target
See all BAX AntibodiesReactivity
Host
Clonality
Conjugate
Application
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Binding Specificity
- N-Term
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Specificity
- Detects endogenous levels of total Bax protein independent of phosphorylation. The antibody does not cross-react with other Bcl-2 family members. Species cross-reactivity: Human, monkey, mouse and rat.
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Purification
- Protein A purified
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Immunogen
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Synthetic peptide (KLH coupled) corresponding to the N-terminal residues of human Bax.
Type of Immunogen: Synthetic peptide -
Isotype
- IgG
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Application Notes
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Approved: ELISA, IP (1:50), WB (1:1000)
Usage: Suitable for use in ELISA, Western Blot and Immunoprecipitation. Immunoprecipitation: 1:50. Western Blot: 1:1000. Note: incubate membrane with diluted antibody in 5 % nonfat dry milk, 1X TBS, 0.1 % Tween-20 at 4?C with gentle shaking, overnight. -
Comment
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Target Species of Antibody: Human
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Restrictions
- For Research Use only
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Format
- Liquid
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Concentration
- Lot specific
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Buffer
- 10 mM HEPES, pH 7.5, 150 mM sodium chloride, 0.1 mg/mL BSA, 50 % glycerol. No preservative added.
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Preservative
- Without preservative
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Handling Advice
- Avoid repeat freeze-thaw cycles.
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Storage
- 4 °C,-20 °C
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Storage Comment
- Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
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- BAX (BCL2-Associated X Protein (BAX))
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Alternative Name
- BAX
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Background
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Name/Gene ID: BAX
Family: Apoptosis
Synonyms: BAX, BCL2-associated X protein, Bcl2-L-4, Apoptosis regulator BAX, Bcl-2-like protein 4, BCL2L4 -
Gene ID
- 581
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UniProt
- Q07812
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Pathways
- p53 Signaling, PI3K-Akt Signaling, Apoptosis, Caspase Cascade in Apoptosis, Positive Regulation of Endopeptidase Activity, Unfolded Protein Response
Target
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