Choline Acetyltransferase antibody

Catalog No. ABIN224453

The Mouse Monoclonal anti-Choline Acetyltransferase antibody is suitable to detect Choline Acetyltransferase in samples from Human, Rat and Pig. It has been validated for WB, IHC, ELISA and IHC (pfa).

Quick Overview for Choline Acetyltransferase antibody (ABIN224453)

Target: Choline Acetyltransferase (CHAT) (Choline O-Acetyltransferase (CHAT))

Reactivity: Human, Rat, Pig

Host: Mouse

Clonality: Monoclonal

Conjugate: This Choline Acetyltransferase antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunohistochemistry (Paraformaldehyde) (IHC (pfa))

Clone: 1-B3-9B3

Product Details anti-Choline Acetyltransferase Antibody

Specificity: Recognizes choline acetyltransferase (ChAT)-positive cells. Species cross-reactivity: Human, Rat and Porcine.

Purification: Purified

Immunogen: Highly purified ChAT obtained from porcine brain

Isotype: IgG1

Application Details

Application Notes: Approved: ELISA, IHC, IHC-Pfa (10 - 20 μg/mL), WB

Usage: Fixation: a) Human tissue: Fix by placing fresh (5-8 h post mortem) tissue in a 1:1 solution of 4 % paraformaldehyde/10 % formaldehyde for 1 week at 4 °C. Divide into tissue blocks (basal forebrain, septum and basal ganglia). Incubate the blocks in sucrose solutions of increasing concentration (10 %, 20 % and 30 %). Prepare 50um sections and store in a cryoprotective solution at -15 °C (3).b) Rat tissue: Brain and spinal cord marrow tissue (transcardial perfusion). Perform a transcardial perfusion initially with oxygen-saturated heparin solution (II) (100-200ml) and subsequently with paraformaldehyde/picric acid solution (III) (400ml). Carry out immediate tissue preparation and fix for a further 30 minutes in the same solution. Wash out the fixative with several fresh quantities of Buffer A. Prepare 50um sections.

Comment:

Target Species of Antibody: Pig

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.6, 0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: avoid freeze thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles.

Target Details for Choline Acetyltransferase

Target: Choline Acetyltransferase (CHAT) (Choline O-Acetyltransferase (CHAT))

Alternative Name: CHAT

Background: Name/Gene ID: CHAT

Synonyms: CHAT, Choline acetylase, Choline acetyltransferase, Choline O-acetyltransferase, CMS1A2, CHOACTASE, CMS1A

Gene ID: 1103

UniProt: P28329

Pathways: Skeletal Muscle Fiber Development