p300 antibody

Catalog No. ABIN224862

The Mouse Monoclonal anti-p300 antibody (ABIN224862) specifically detects p300 in WB, IHC, IF, IP and IHC (p). The antibody is reactive with Human samples.

Quick Overview for p300 antibody (ABIN224862)

Target: p300 (EP300) (E1A Binding Protein P300 (EP300))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This p300 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-p300 Antibody

Specificity: Recognizes human p300. Does not react with mouse or rat p300.

Purification: Protein A purified

Immunogen: Recombinant human p300 protein.

Type of Immunogen: Recombinant protein

Isotype: IgG1

Application Details

Application Notes: Approved: IF (1 - 5 μg/mL), IHC, IHC-P (1 - 5 μg/mL), IP, WB (1 μg/mL)

Usage: Suitable for use in Immunofluorescence, Western Blot, Immunohistochemistry and Immunoprecipitation. Immunofluorescence: 1-5 μg/mL. Immunohistochemistry: 1-5 μg/mL, Staining of formalin fixed, paraffin embedded tissue requires HIER pretreatment. Immunoprecipitation: 1 μg/sample, Autoradiographic detection. Western Blot: 1 μg/mL, Chemiluminescence detection. Positive control: A431, HeLa or ML-1 cells.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.4, 0.1 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for p300

Target: p300 (EP300) (E1A Binding Protein P300 (EP300))

Alternative Name: EP300 / p300

Background: Name/Gene ID: EP300

Synonyms: EP300, E1A binding protein p300, Histone acetyltransferase p300, KAT3B, p300 HAT, E1A-associated protein p300, E1A-binding protein, 300kD, p300, RSTS2

Gene ID: 2033

UniProt: Q09472

Pathways: p53 Signaling, Notch Signaling, Interferon-gamma Pathway, Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Regulation of Lipid Metabolism by PPARalpha, Regulation of Muscle Cell Differentiation, Regulation of Cell Size