Phospholipase C gamma 1 antibody

Catalog No. ABIN226218

This anti-Phospholipase C gamma 1 antibody is a Mouse Monoclonal antibody detecting Phospholipase C gamma 1 in WB, ELISA, ICC and IP. Suitable for Human, Mouse, Rat, Cow and Rabbit.

Quick Overview for Phospholipase C gamma 1 antibody (ABIN226218)

Target: Phospholipase C gamma 1 (PLCG1) (phospholipase C, gamma 1 (PLCG1))

Reactivity: Human, Mouse, Rat, Cow, Rabbit

Host: Mouse

Clonality: Monoclonal

Conjugate: This Phospholipase C gamma 1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunocytochemistry (ICC), Immunoprecipitation (IP)

Product Details anti-Phospholipase C gamma 1 Antibody

Specificity: Recognizes bovine PLC g-1 at 135kD. Reacts with some non-PLC g-1 SH-3 containing proteins, including nck (47kD). Species cross-reactivity: rabbit, rat, mouse and human.

Purification: Protein G purified

Immunogen: Purified soluble phospholipase C (PLC) g-1 from bovine brain.

Type of Immunogen: Purified protein

Isotype: IgG

Application Details

Application Notes: Approved: ELISA, ICC (5 - 10 μg/mL), IP, WB (0.5 - 2 μg/mL)

Usage: Suitable for use in ELISA, Immunoprecipitation, Western Blot and Immunocytochemistry. Western Blot: 0.5-2 μg/mL detects PLCg1 in human A431 carcinoma cells as well as rabbit and bovine brain cytosol preparations. Immunoprecipitation: 4 μg Immunoprecipitates PLCg1 from non-stimulated A431 cell lysates and from rabbit brain cytosol preparation. Immunocytochemistry: 5-10 μg/mL detects PLCg1 on ethanol-acetic acid (95:5) fixed A431 cells.

Comment:

Target Species of Antibody: Bovine

Restrictions: For Research Use only

Handling

Format: Liquid

Reconstitution: dH2O, 40-50 % glycerol

Concentration: Lot specific

Buffer: 0.1 M Tris-glycine,  pH 7.4, 150 mM sodium chloride, 0.05 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C
Long term: Add glycerol (40-50%) -20°C.

Target Details for Phospholipase C gamma 1

Target: Phospholipase C gamma 1 (PLCG1) (phospholipase C, gamma 1 (PLCG1))

Alternative Name: PLCG1

Background: Name/Gene ID: PLCG1
Family: Phospholipase

Synonyms: PLCG1, Phospholipase C, gamma 1, Phospholipase C-148, Phosphoinositidase C, Phospholipase C-II, Inositoltrisphosphohydrolase, PLC-gamma-1, PLC1, PLC-148, PLC-II, PLC148, NCKAP3, Phospholipase C-gamma-1, PLCgamma1

Gene ID: 5335

UniProt: P19174

Pathways: RTK Signaling, WNT Signaling, TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Thyroid Hormone Synthesis, Inositol Metabolic Process, Myometrial Relaxation and Contraction, Regulation of Muscle Cell Differentiation, Regulation of G-Protein Coupled Receptor Protein Signaling, Skeletal Muscle Fiber Development, G-protein mediated Events, Signaling Events mediated by VEGFR1 and VEGFR2, Interaction of EGFR with phospholipase C-gamma, VEGFR1 Specific Signals, VEGF Signaling