Phospholipase C gamma 1 antibody
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- Target See all Phospholipase C gamma 1 (PLCG1) Antibodies
- Phospholipase C gamma 1 (PLCG1) (phospholipase C, gamma 1 (PLCG1))
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Reactivity
- Human, Mouse, Rat, Cow, Rabbit
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Host
- Mouse
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Clonality
- Monoclonal
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Conjugate
- This Phospholipase C gamma 1 antibody is un-conjugated
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Application
- Western Blotting (WB), ELISA, Immunocytochemistry (ICC), Immunoprecipitation (IP)
- Specificity
- Recognizes bovine PLC g-1 at 135kD. Reacts with some non-PLC g-1 SH-3 containing proteins, including nck (47kD). Species cross-reactivity: rabbit, rat, mouse and human.
- Purification
- Protein G purified
- Immunogen
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Purified soluble phospholipase C (PLC) g-1 from bovine brain.
Type of Immunogen: Purified protein - Isotype
- IgG
- Top Product
- Discover our top product PLCG1 Primary Antibody
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- Application Notes
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Approved: ELISA, ICC (5 - 10 μg/mL), IP, WB (0.5 - 2 μg/mL)
Usage: Suitable for use in ELISA, Immunoprecipitation, Western Blot and Immunocytochemistry. Western Blot: 0.5-2 μg/mL detects PLCg1 in human A431 carcinoma cells as well as rabbit and bovine brain cytosol preparations. Immunoprecipitation: 4 μg Immunoprecipitates PLCg1 from non-stimulated A431 cell lysates and from rabbit brain cytosol preparation. Immunocytochemistry: 5-10 μg/mL detects PLCg1 on ethanol-acetic acid (95:5) fixed A431 cells. - Comment
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Target Species of Antibody: Bovine
- Restrictions
- For Research Use only
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- Format
- Liquid
- Reconstitution
- dH2O, 40-50 % glycerol
- Concentration
- Lot specific
- Buffer
- 0.1 M Tris-glycine, pH 7.4, 150 mM sodium chloride, 0.05 % sodium azide.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- 4 °C,-20 °C
- Storage Comment
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Short term: 4°C
Long term: Add glycerol (40-50%) -20°C.
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- Target
- Phospholipase C gamma 1 (PLCG1) (phospholipase C, gamma 1 (PLCG1))
- Alternative Name
- PLCG1 (PLCG1 Products)
- Synonyms
- NCKAP3 antibody, PLC-II antibody, PLC1 antibody, PLC148 antibody, PLCgamma1 antibody, plc1 antibody, nckap3 antibody, plc-ii antibody, plc148 antibody, plcgamma1 antibody, PPLCA antibody, AI894140 antibody, Cded antibody, Plc-1 antibody, Plc-gamma1 antibody, Plcg-1 antibody, fj46f04 antibody, wu:fj46f04 antibody, wu:fj57a05 antibody, phospholipase C gamma 1 antibody, phospholipase C gamma 1 L homeolog antibody, 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase gamma-1 antibody, phospholipase C, gamma 1 antibody, PLCG1 antibody, plcg1.L antibody, LOC100633163 antibody, Plcg1 antibody, plcg1 antibody
- Background
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Name/Gene ID: PLCG1
Family: Phospholipase
Synonyms: PLCG1, Phospholipase C, gamma 1, Phospholipase C-148, Phosphoinositidase C, Phospholipase C-II, Inositoltrisphosphohydrolase, PLC-gamma-1, PLC1, PLC-148, PLC-II, PLC148, NCKAP3, Phospholipase C-gamma-1, PLCgamma1 - Gene ID
- 5335
- UniProt
- P19174
- Pathways
- RTK Signaling, WNT Signaling, TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Thyroid Hormone Synthesis, Inositol Metabolic Process, Myometrial Relaxation and Contraction, Regulation of Muscle Cell Differentiation, Regulation of G-Protein Coupled Receptor Protein Signaling, Skeletal Muscle Fiber Development, G-protein mediated Events, Signaling Events mediated by VEGFR1 and VEGFR2, Interaction of EGFR with phospholipase C-gamma, VEGFR1 Specific Signals, VEGF Signaling
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