MIF antibody

Catalog No. ABIN7473237

This Mouse Monoclonal antibody specifically detects MIF in WB and ELISA. It exhibits reactivity toward Human.

Quick Overview for MIF antibody (ABIN7473237)

Target: MIF (Macrophage Migration Inhibitory Factor (Glycosylation-Inhibiting Factor) (MIF))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This MIF antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Clone: 2AR3

Product Details anti-MIF Antibody

Specificity: Recognizes recombinant and natural human Migration Inhibitory Factor (MIF). Based on direct ELISA, shows no cross-reactivity with most cytokines.

Purification: Protein G purified

Immunogen: Recombinant human MIF protein expressed in E. coli.

Type of Immunogen: Recombinant protein

Isotype: IgG1

Application Details

Application Notes: Approved: ELISA (4 μg/mL), WB (1 - 2 μg/mL)

Usage: Suitable for use in ELISA and Western Blot. Western Blot: 1-2 μg/mL. Detection limit for is ~5 ng/lane under non-reducing and reducing conditions.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 1 mL sterile PBS

Concentration: Lot specific

Buffer: Lyophilized from PBS,  pH 7.4, 5 % trehalose

Storage: -20 °C

Storage Comment: Short term: -20°C
Long term: -20°C.

Target Details for MIF

Target: MIF (Macrophage Migration Inhibitory Factor (Glycosylation-Inhibiting Factor) (MIF))

Alternative Name: MIF

Background: Name/Gene ID: MIF

Synonyms: MIF, GLIF, L-dopachrome tautomerase, L-dopachrome isomerase, MMIF, Phenylpyruvate tautomerase

Gene ID: 4282

UniProt: P14174

Pathways: Regulation of Systemic Arterial Blood Pressure by Hormones, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Regulation of Carbohydrate Metabolic Process, Feeding Behaviour, Smooth Muscle Cell Migration, Negative Regulation of intrinsic apoptotic Signaling