CDK4 antibody (AA 1-20)

Catalog No. ABIN227544

This anti-CDK4 antibody is a Mouse Monoclonal antibody detecting CDK4 in WB, IP, IF and Func. Suitable for Human, Mouse, Rat and Pig.

Quick Overview for CDK4 antibody (AA 1-20) (ABIN227544)

Target: CDK4 (Cyclin-Dependent Kinase 4 (CDK4))

Reactivity: Human, Mouse, Rat, Pig

Host: Mouse

Clonality: Monoclonal

Conjugate: This CDK4 antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunofluorescence (IF), Functional Studies (Func)

Product Details anti-CDK4 Antibody

Binding Specificity: AA 1-20

Specificity: Recognizes p34 (34kD). Does not cross-react with other cdks. Species cross-reactivity: Human, mouse, rat and porcine.

Purification: Protein G purified

Immunogen: Purified recombinant cdk4 protein. Cellular Localization: Nuclear and cytoplasmic. Epitope: aa1-20.

Type of Immunogen: Recombinant protein

Isotype: IgG1 kappa

Application Details

Application Notes: Approved: Func, IF, IP, WB (1 - 2 μg/mL)

Usage: Suitable for use in Immunofluorescence, Immunoprecipitation, Western Blot and Kinase assays. Immunoprecipitation Native only, coprecipitates cyclin D's: 2 μg/mg protein lysate. Use protein G. Western Blot: 1-2 μg/mL for 2 hours at RT. Positive control: MAD109, NIH3T3, LS174T, and MCF-7 cells.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.4, 0.2 % BSA, 0.1 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C
Long term: Add glycerol (40-50%) -20°C.

Target Details for CDK4

Target: CDK4 (Cyclin-Dependent Kinase 4 (CDK4))

Alternative Name: CDK4

Background: Name/Gene ID: CDK4
Subfamily: CDC2/CDK
Family: Protein Kinase

Synonyms: CDK4, Cell division protein kinase 4, Crk3, Cyclin-dependent kinase 4, CMM3, p34/cdk4, PSK-J3

Gene ID: 1019

UniProt: P11802

Pathways: Cell Division Cycle, Mitotic G1-G1/S Phases, Regulation of Cell Size