Insulin Receptor antibody (Exon 7)

Catalog No. ABIN228599

The Mouse Monoclonal anti-Insulin Receptor antibody is suitable to detect Insulin Receptor in samples from Human. It has been validated for ELISA, IP and Func.

Quick Overview for Insulin Receptor antibody (Exon 7) (ABIN228599)

Target: Insulin Receptor (INSR)

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Insulin Receptor antibody is un-conjugated

Application: ELISA, Immunoprecipitation (IP), Functional Studies (Func)

Clone: 83-14

Product Details anti-Insulin Receptor Antibody

Binding Specificity: AA 469-592, Exon 7

Specificity: Recognizes human Insulin receptor, alpha subunit. Inhibits insulin binding (~80 % ) and mimics insulin action. Weakly reacts with bovine, porcine and sheep. Does not react with rabbit, mouse and rat.

Purification: Protein A purified

Immunogen: IM-9 lymphocytes followed by purified insulin receptor. MW of Antigen: 135kD. Epitope: aa469-592 (exon 7/8). Cellular Localization: Cell membrane.

Type of Immunogen: Cells

Isotype: IgG2a kappa

Application Details

Application Notes: Approved: ELISA, Func, IP

Usage: Suitable for use in ELISA, Immunoprecipitation, Tyrosine Kinase Assay and Inhibition of insulin binding. May also be used as insulin-like agonist. ELISA: Both as capture and detection. Immunoprecipitation (Use Protein G): 2 μg/mg protein lysate. Tyrosine Kinase Assay: Antibody-mediated capture on microtiter plates. Positive control: IM-9 lymphocyte cells and Placenta. The applications listed have been tested for the unmodified form of this product. Other forms have not been tested.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: 10 mM PBS,  pH 7.4. Glycerol free.

Preservative: Azide free

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for Insulin Receptor

Target: Insulin Receptor (INSR)

Alternative Name: INSR / Insulin Receptor

Background: Name/Gene ID: INSR
Subfamily: Insulin receptor
Family: Protein Kinase

Synonyms: INSR, CD220, ILP receptor, Insulin receptor, IR, HHF5, CD220 antigen

Gene ID: 3643

UniProt: P06213

Pathways: NF-kappaB Signaling, RTK Signaling, AMPK Signaling, Carbohydrate Homeostasis, Regulation of Cell Size, Regulation of Carbohydrate Metabolic Process, Growth Factor Binding, Negative Regulation of Transporter Activity