MEK1 antibody (pSer218)
Quick Overview for MEK1 antibody (pSer218) (ABIN228872)
Target
See all MEK1 (MAP2K1) AntibodiesReactivity
Host
Clonality
Conjugate
Application
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Binding Specificity
- pSer218
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Specificity
- Recognizes human MEK 1 and MEK 2, when phosphorylated at serine 218 and 222. MEK 1 and MEK 2 are also known as MAP2K1 and MAP2K2, respectively. Species cross-reactivity: mouse. Species sequence homology: rat, bovine, canine, chicken, xenopus, zebrafish, monkey.
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Purification
- Affinity purified
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Immunogen
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Synthetic phosphopeptide corresponding to an amino acid sequence which includes phosphorylated serine 218 and 222 within MEK1 and 2.
Type of Immunogen: Synthetic phospho-peptide -
Isotype
- IgG
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anti-Mitogen-Activated Protein Kinase Kinase 1 (MAP2K1) (AA 2-150) antibody
MAP2K1 Reactivity: Human ELISA, IHC (p), IF (cc), IF (p), FACS, IHC (fro) Host: Rabbit Polyclonal unconjugated
anti-Mitogen-Activated Protein Kinase Kinase 1 (MAP2K1) antibodyMAP2K1 Reactivity: Human, Mouse, Rat, Dog, Monkey WB, IHC, IF, IHC (p) Host: Mouse Monoclonal 3H3 unconjugated
anti-Mitogen-Activated Protein Kinase Kinase 1 (MAP2K1) antibodyMAP2K1 Reactivity: Human, Mouse, Rat, Dog, Monkey WB, IHC, IF, IHC (p) Host: Mouse Monoclonal 2H3 unconjugated
anti-Mitogen-Activated Protein Kinase Kinase 1 (MAP2K1) (AA 1-100) antibodyMAP2K1 Reactivity: Human WB, IF Host: Rabbit Polyclonal unconjugated
anti-Mitogen-Activated Protein Kinase Kinase 1 (MAP2K1) (pSer298) antibodyKD Validated MAP2K1 Reactivity: Human WB, ICC, FACS Host: Rabbit Monoclonal 24GB285 unconjugated Recombinant Antibody
anti-Mitogen-Activated Protein Kinase Kinase 1 (MAP2K1) antibodyMAP2K1 Reactivity: Human, Mouse, Rat, Dog, Monkey WB, IHC, IF, IHC (p) Host: Mouse Monoclonal 3B4 unconjugated
anti-Mitogen-Activated Protein Kinase Kinase 1 (MAP2K1) antibodyKD Validated MAP2K1 Reactivity: Human WB, FACS Host: Rabbit Monoclonal 23GB2475 unconjugated Recombinant Antibody
anti-Mitogen-Activated Protein Kinase Kinase 1 (MAP2K1) (pSer221) antibodyMAP2K1 Reactivity: Human WB, IHC, ELISA, IF Host: Rabbit Polyclonal unconjugated
anti-Mitogen-Activated Protein Kinase Kinase 1 (MAP2K1) (N-Term) antibodyMAP2K1 Reactivity: Human, Mouse, Rat, Xenopus laevis WB, IHC, IF, IHC (p) Host: Rabbit Polyclonal unconjugated
anti-Mitogen-Activated Protein Kinase Kinase 1 (MAP2K1) (pThr292) antibodyKD Validated MAP2K1 Reactivity: Human WB, ICC, FACS Host: Rabbit Monoclonal 24GB305 unconjugated Recombinant Antibody
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Application Notes
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Approved: WB (1:1000)
Usage: Suitable for use in Western Blot. Western Blot: 1:1000. Recognizes a band of ~45kD in Western Blots of serum stimulated NIH3T3 cell lysates. Positive control: NIH 3T3 cell lysate. -
Comment
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Target Species of Antibody: Human
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Restrictions
- For Research Use only
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Format
- Liquid
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Concentration
- Lot specific
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Buffer
- 10 mM HEPES, pH 7.5, 0.01 % BSA, 0.1 % sodium azide, 50 % glycerol.
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Preservative
- Sodium azide
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Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Handling Advice
- Avoid repeat freeze-thaw cycles.
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Storage
- 4 °C,-20 °C
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Storage Comment
- Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
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- MEK1 (MAP2K1) (Mitogen-Activated Protein Kinase Kinase 1 (MAP2K1))
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Alternative Name
- MAP2K1 / MEK1
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Background
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Name/Gene ID: MAP2K1
Subfamily: MAP2K
Family: Protein Kinase
Synonyms: MAP2K1, ERK activator kinase 1, MAPK/ERK kinase 1, MAPKK 1, MKK1, PRKMK1, MEK 1, MAP kinase kinase 1, MAPKK1, MEK1 -
Gene ID
- 5604
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UniProt
- Q02750
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Pathways
- MAPK Signaling, RTK Signaling, Interferon-gamma Pathway, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Toll-Like Receptors Cascades, Autophagy, Signaling of Hepatocyte Growth Factor Receptor, BCR Signaling
Target
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