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c-MET antibody (pTyr1234, Tyr1235)

This anti-c-MET antibody is a Rabbit Polyclonal antibody detecting c-MET in WB, ELISA, ICC and IP. Suitable for Human.
Catalog No. ABIN228894

Quick Overview for c-MET antibody (pTyr1234, Tyr1235) (ABIN228894)

Target

See all c-MET (MET) Antibodies
c-MET (MET) (Met Proto-Oncogene (MET))

Reactivity

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Human

Host

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Rabbit

Clonality

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Polyclonal

Conjugate

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This c-MET antibody is un-conjugated

Application

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Western Blotting (WB), ELISA, Immunocytochemistry (ICC), Immunoprecipitation (IP)
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    pTyr1234, Tyr1235

    Specificity

    Detects Met only when phosphorylated at tyrosine 1234/1235 (Mr 145kD). This antibody cross-reacts slightly with activated PDGF, insulin and FGF receptors, but it does not cross-react with other tyrosine-phosphorylated proteins. Species cross-reactivity: Human, mouse and monkey. Species sequence homology: rat 100 % .

    Purification

    Immunoaffinity purified

    Immunogen

    Synthetic phosphopeptide (KLH coupled) corresponding to residues surrounding Tyr1234/1235 of human Met.

    Type of Immunogen: Synthetic phospho-peptide

    Isotype

    IgG
  • Application Notes

    Approved: ELISA, ICC (1:50), IP (1:50), WB (1:1000)

    Usage: Suitable for use in ELISA, Western Blot, Immunoprecipitation and Immunocytochemistry. Western Blot: 1:1000. Incubate membrane with diluted antibody in 5 % BSA, 1X TBS, 0.1 % Tween-20 at 4?C with gentle shaking, overnight. Immunoprecipitation: 1:50.

    Comment

    Target Species of Antibody: Human

    Restrictions

    For Research Use only
  • Format

    Liquid

    Concentration

    Lot specific

    Buffer

    10 mM sodium HEPES,  pH 7.5, 150 mM sodium chloride, 0.1 mg/mL BSA, 50 % glycerol.

    Handling Advice

    Avoid repeated freezing and thawing.

    Storage

    4 °C,-20 °C

    Storage Comment

    4°C or -20°C, Avoid freeze-thaw cycles.
  • Target

    c-MET (MET) (Met Proto-Oncogene (MET))

    Alternative Name

    c-Met

    Background

    Name/Gene ID: MET
    Subfamily: HGF Receptor/MET
    Family: Protein Kinase

    Synonyms: MET, AUTS9, C-Met, HGF/SF receptor, HGFR, Met kinase, Proto-oncogene c-Met, Scatter factor receptor, SF receptor, HGF receptor, RCCP2, Tyrosine-protein kinase Met

    Gene ID

    4233

    UniProt

    P08581

    Pathways

    RTK Signaling, Carbohydrate Homeostasis, Synaptic Membrane, Signaling of Hepatocyte Growth Factor Receptor
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