c-MET antibody (pTyr1234, Tyr1235)
Quick Overview for c-MET antibody (pTyr1234, Tyr1235) (ABIN228894)
Target
See all c-MET (MET) AntibodiesReactivity
Host
Clonality
Conjugate
Application
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Binding Specificity
- pTyr1234, Tyr1235
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Specificity
- Detects Met only when phosphorylated at tyrosine 1234/1235 (Mr 145kD). This antibody cross-reacts slightly with activated PDGF, insulin and FGF receptors, but it does not cross-react with other tyrosine-phosphorylated proteins. Species cross-reactivity: Human, mouse and monkey. Species sequence homology: rat 100 % .
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Purification
- Immunoaffinity purified
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Immunogen
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Synthetic phosphopeptide (KLH coupled) corresponding to residues surrounding Tyr1234/1235 of human Met.
Type of Immunogen: Synthetic phospho-peptide -
Isotype
- IgG
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Application Notes
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Approved: ELISA, ICC (1:50), IP (1:50), WB (1:1000)
Usage: Suitable for use in ELISA, Western Blot, Immunoprecipitation and Immunocytochemistry. Western Blot: 1:1000. Incubate membrane with diluted antibody in 5 % BSA, 1X TBS, 0.1 % Tween-20 at 4?C with gentle shaking, overnight. Immunoprecipitation: 1:50. -
Comment
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Target Species of Antibody: Human
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Restrictions
- For Research Use only
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Format
- Liquid
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Concentration
- Lot specific
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Buffer
- 10 mM sodium HEPES, pH 7.5, 150 mM sodium chloride, 0.1 mg/mL BSA, 50 % glycerol.
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Handling Advice
- Avoid repeated freezing and thawing.
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Storage
- 4 °C,-20 °C
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Storage Comment
- 4°C or -20°C, Avoid freeze-thaw cycles.
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- c-MET (MET) (Met Proto-Oncogene (MET))
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Alternative Name
- c-Met
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Background
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Name/Gene ID: MET
Subfamily: HGF Receptor/MET
Family: Protein Kinase
Synonyms: MET, AUTS9, C-Met, HGF/SF receptor, HGFR, Met kinase, Proto-oncogene c-Met, Scatter factor receptor, SF receptor, HGF receptor, RCCP2, Tyrosine-protein kinase Met -
Gene ID
- 4233
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UniProt
- P08581
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Pathways
- RTK Signaling, Carbohydrate Homeostasis, Synaptic Membrane, Signaling of Hepatocyte Growth Factor Receptor
Target
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