RPS6KB1 antibody (C-Term)

Catalog No. ABIN229044

The Rabbit Polyclonal anti-RPS6KB1 antibody (ABIN229044) specifically detects RPS6KB1 in WB, ELISA and IP. The antibody is reactive with Human, Mouse, Rat and Monkey samples.

Quick Overview for RPS6KB1 antibody (C-Term) (ABIN229044)

Target: RPS6KB1 (Ribosomal Protein S6 Kinase, 70kDa, Polypeptide 1 (RPS6KB1))

Reactivity: Human, Mouse, Rat, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RPS6KB1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunoprecipitation (IP)

Product Details anti-RPS6KB1 Antibody

Binding Specificity: C-Term

Specificity: Recognizes total levels of endogenous human p70 S6 kinase protein at 70kD. Also recognizes p85 S6 kinase at 85kD. Species cross-reactivity: mouse, rat and monkey.

Purification: Protein A purified

Immunogen: Synthetic peptide derived from the sequence near the C terminus of human p70 S6 kinase (KLH).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: ELISA, IP (1:50), WB (1:1000)

Usage: Suitable for use in ELISA, Western Blot and Immunoprecipitation. Western Blot: 1:1000, Incubate membrane with diluted antibody in 5 % BSA, 1X TBS, 0.1 % Tween-20 at 4?C with gentle shaking, overnight. Immunoprecipitation: 1:50.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: 10 mM HEPES,  pH 7.5, 150 mM sodium chloride, 0.1 mg/mL BSA, 50 % glycerol. No preservative added.

Preservative: Without preservative

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for RPS6KB1

Target: RPS6KB1 (Ribosomal Protein S6 Kinase, 70kDa, Polypeptide 1 (RPS6KB1))

Alternative Name: RPS6KB1 / P70S6K / S6K

Background: Name/Gene ID: RPS6KB1
Subfamily: P70 RSK
Family: Protein Kinase

Synonyms: RPS6KB1, 70 kDa S6 kinase, p70 S6KA, p70-alpha, p70-S6K, p70 S6 kinase alpha, p70s6 kinase, S6K-beta-1, STK14A, p70 ribosomal S6 kinase, p70 S6 kinase, alpha 1, p70 S6 kinase, alpha 2, p70 S6K-alpha, p70-S6K 1, p70S6K, p70S6K1, PS6K, Ribosomal protein S6 kinase I, S6K, S6K1, p70 ribosomal S6 kinase alpha, p70(S6K)-alpha

Gene ID: 6198

UniProt: P23443

Pathways: PI3K-Akt Signaling, RTK Signaling, AMPK Signaling, Regulation of Cell Size, Skeletal Muscle Fiber Development, Feeding Behaviour, G-protein mediated Events, Smooth Muscle Cell Migration, Interaction of EGFR with phospholipase C-gamma, Warburg Effect