Crk antibody (pTyr221)

Catalog No. ABIN229989

Product Details anti-Crk Antibody

Target: Crk (CRK) (V-Crk Sarcoma Virus CT10 Oncogene Homolog (Avian) (CRK))

Binding Specificity: pTyr221

Reactivity: Human, Hamster

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Crk antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Specificity: Detects endogenous levels of CrkII only when phosphorylated at tyrosine 221 at 42kD. Cross-reactivity: Tyr207 phosphorylated CrkL. Does not cross-react with other tyrosine-phosphorylated proteins. Species cross-reactivity: Human and hamster. Species sequence homology: mouse and rat 100 % .

Purification: Immunoaffinity purified

Immunogen: Synthetic phosphopeptide (KLH coupled) corresponding to residues surrounding Tyr221 of human CrkII.

Type of Immunogen: Synthetic phospho-peptide

Isotype: IgG

Application Details

Application Notes: Approved: ELISA, WB (1:1000)

Usage: Suitable for use in ELISA and Western Blot. Western Blot: 1:1000. Note: for Western Blots incubate membrane with diluted antibody in 5 % BSA, 1X TBS, and 0.1 % Tween-20 at 4?C with gentle shaking overnight.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: 10 mM sodium HEPES,  pH 7.5, 150 mM sodium chloride, 0.1 mg/mL BSA, 50 % glycerol.

Target Details for Crk

Target: Crk (CRK) (V-Crk Sarcoma Virus CT10 Oncogene Homolog (Avian) (CRK))

Alternative Name: CRK (CRK Products)

Synonyms: CG1587 antibody, CRK antibody, D-CRK antibody, D-Crk antibody, DCrk antibody, Dcrk antibody, Dmel\\CG1587 antibody, crk antibody, dCRK antibody, dCrk antibody, fb34h10 antibody, fc05a01 antibody, fi19g05 antibody, wu:fb34h10 antibody, wu:fc05a01 antibody, wu:fc54a04 antibody, wu:fi19g05 antibody, zgc:100936 antibody, CRKII antibody, p38 antibody, Crko antibody, crk2 antibody, crkII antibody, v-crk antibody, P38C-CRK antibody, Crk-I antibody, Crk-II antibody, Crk-III antibody, Crk3 antibody, CrkIII antibody, c-Crk antibody, Crk oncogene antibody, v-crk avian sarcoma virus CT10 oncogene homolog antibody, CRK proto-oncogene, adaptor protein antibody, v-crk avian sarcoma virus CT10 oncogene homolog L homeolog antibody, v-crk sarcoma virus CT10 oncogene homolog antibody, Crk antibody, crk antibody, CRK antibody, crk.L antibody

Background: Name/Gene ID: CRK

Synonyms: CRK, Adapter molecule crk, CRKII, Proto-oncogene c-Crk, p38

Gene ID: 1398

UniProt: P46108

Pathways: Neurotrophin Signaling Pathway, CXCR4-mediated Signaling Events, Signaling of Hepatocyte Growth Factor Receptor