CHEK1 antibody (pSer345)

Catalog No. ABIN232760

The Rabbit Polyclonal anti-CHEK1 antibody has been validated for WB. It is suitable to detect CHEK1 in samples from Human, Mouse, Rat and Monkey.

Quick Overview for CHEK1 antibody (pSer345) (ABIN232760)

Target: CHEK1 (Checkpoint Kinase 1 (CHEK1))

Reactivity: Human, Mouse, Rat, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CHEK1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-CHEK1 Antibody

Binding Specificity: pSer345

Specificity: Detects endogenous levels of Chk1 only when activated by phosphorylation at serine 345. Does not recognize Chk1 phosphorylated at other sites. Species cross-reactivity: Monkey, mouse and rat.

Purification: Protein A purified

Immunogen: Synthetic phosphopeptide (KLH coupled) corresponding to residues surrounding Ser345 of human Chk1.

Type of Immunogen: Synthetic phospho-peptide

Isotype: IgG

Application Details

Application Notes: Approved: WB (1:1000)

Usage: Western Blot: 1:1000, incubate membrane with diluted antibody in 5 % BSA, 1X TBS, 0.1 % Tween-20 at 4?C with gentle shaking, overnight. Flow Cytometry: 1:50.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: 10 mM HEPES,  pH 7.5, 150 mM sodium chloride, 0.1 mg/mL BSA, 50 % glycerol. No preservative added.

Preservative: Without preservative

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for CHEK1

Target: CHEK1 (Checkpoint Kinase 1 (CHEK1))

Alternative Name: CHEK1 / CHK1

Background: Name/Gene ID: CHEK1
Subfamily: NIM1
Family: Protein Kinase

Synonyms: CHEK1, CHK1 checkpoint homolog, Cell cycle checkpoint kinase, Checkpoint kinase 1, CHK1, Chk1-S, Protein kinase chk1, Checkpoint kinase-1

Gene ID: 1111

UniProt: O14757

Pathways: p53 Signaling, Apoptosis, Cell Division Cycle, DNA Damage Repair