EGFR antibody (pTyr1068)

Catalog No. ABIN232925

The Rabbit Polyclonal anti-EGFR antibody is suitable to detect EGFR in samples from Human and Rat. It has been validated for WB, IHC, ELISA and IHC (p).

Quick Overview for EGFR antibody (pTyr1068) (ABIN232925)

Target: EGFR (Epidermal Growth Factor Receptor (EGFR))

Reactivity: Human, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This EGFR antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-EGFR Antibody

Binding Specificity: pTyr1068

Specificity: Recognizes endogenous levels of human EGF receptor at ~175kD only when phosphorylated at Tyr1068 at 175kD. The antibody may cross-react with other activated EGF receptor family members (e. g., ErbB2), and cross-reacts slightly with activated PDGF receptor.

Purification: Immunoaffinity purified

Immunogen: Synthetic phosphopeptide corresponding to residues surrounding Tyr1068 of human EGF receptor (KLH).

Type of Immunogen: Synthetic phospho-peptide

Isotype: IgG

Application Details

Application Notes: Approved: ELISA, IHC, IHC-P, WB (1:1000)

Usage: Suitable for use in ELISA, Western Blot and Immunohistochemistry. Western Blot: 1:1000. Immunohistochemistry (Paraffin): 1:50-350.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: 10 mM HEPES,  pH 7.5, 150 mM sodium chloride, 0.1 mg/mL BSA, 50 % glycerol. No preservative added.

Preservative: Without preservative

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for EGFR

Target: EGFR (Epidermal Growth Factor Receptor (EGFR))

Alternative Name: EGFR

Background: Name/Gene ID: EGFR
Subfamily: EGF Receptor
Family: Protein Kinase

Synonyms: EGFR, EGFf receptor, ERBB, ERBB1, MENA, PIG61, Proto-oncogene c-ErbB-1, V-erb-b homolog, EGF-R, HER1

Gene ID: 1956

UniProt: P00533

Pathways: NF-kappaB Signaling, RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Stem Cell Maintenance, Hepatitis C, Positive Regulation of Response to DNA Damage Stimulus, Interaction of EGFR with phospholipase C-gamma, Thromboxane A2 Receptor Signaling, EGFR Downregulation, S100 Proteins