LCK antibody (AA 1-225)

Catalog No. ABIN233301

The Mouse Monoclonal anti-LCK antibody (ABIN233301) specifically detects LCK in WB, ELISA, IP and Func. The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for LCK antibody (AA 1-225) (ABIN233301)

Target: LCK (Lymphocyte-Specific Protein tyrosine Kinase (LCK))

Reactivity: Human, Mouse, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This LCK antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunoprecipitation (IP), Functional Studies (Func)

Product Details anti-LCK Antibody

Binding Specificity: AA 1-225

Specificity: Recognizes Lck at 56kD. Species cross-reactivity: Human, mouse and rat.

Purification: Protein A purified

Immunogen: Recombinant protein corresponding to aa1-225 of murine p56lck protein. Cellular Localization: Cell membrane.

Type of Immunogen: Recombinant protein

Isotype: IgG2b kappa

Application Details

Application Notes: Approved: ELISA, Func, IP, WB (1 - 2 μg/mL)

Usage: Suitable for use in ELISA, Immunoprecipitation, Kinase Assay, and Western Blot. Immunoprecipitation: Use Protein A. 2 μg antibody/mg protein lysate. Western Blot: 1-2 μg/mL for 2 hours at RT. Positive control: Jurkat cells.

Comment:

Target Species of Antibody: Mouse

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.4, 0.2 % BSA, 0.1 % sodium azide, 40 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for LCK

Target: LCK (Lymphocyte-Specific Protein tyrosine Kinase (LCK))

Alternative Name: p56lck / LCK

Background: Name/Gene ID: LCK
Subfamily: Src
Family: Protein Kinase

Synonyms: LCK, Lck kinase, LSK, p56-LCK, Pp58lck, Tyrosine-protein kinase Lck, p56lck, Protein YT16, Proto-oncogene Lck, YT16

Gene ID: 3932

UniProt: P06239

Pathways: TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Transition Metal Ion Homeostasis, Positive Regulation of Endopeptidase Activity, CXCR4-mediated Signaling Events, Thromboxane A2 Receptor Signaling