LCK antibody (pTyr505)

Catalog No. ABIN233303

The Rabbit Polyclonal anti-LCK antibody has been validated for WB, IHC, IP and IHC (p). It is suitable to detect LCK in samples from Human and Mouse.

Quick Overview for LCK antibody (pTyr505) (ABIN233303)

Target: LCK (Lymphocyte-Specific Protein tyrosine Kinase (LCK))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This LCK antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-LCK Antibody

Binding Specificity: pTyr505

Specificity: Detects endogenous levels human Lck only when phosphorylated at Tyr505. Can cross-react with phospho-Lyn (Tyr507) due to high sequence homology. Species cross-reactivity: mouse.

Purification: Protein A purified

Immunogen: Synthetic phospho-peptide (KLH coupled) corresponding to residues surrounding Tyr505 of human Lck.

Type of Immunogen: Synthetic phospho-peptide

Isotype: IgG

Application Details

Application Notes: Approved: IHC, IHC-P, IP (1:100), WB (1:1000)

Usage: Western Blot: 1:1000. Immunoprecipitation: 1:100. Immunohistochemistry (Paraffin): 1:50.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: 10 mM HEPES,  pH 7.5, 150 mM sodium chloride, 0.1 mg/mL BSA, 50 % glycerol. No preservative added.

Preservative: Without preservative

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for LCK

Target: LCK (Lymphocyte-Specific Protein tyrosine Kinase (LCK))

Alternative Name: p56lck / LCK

Background: Name/Gene ID: LCK
Subfamily: Src
Family: Protein Kinase

Synonyms: LCK, Lck kinase, LSK, p56-LCK, Pp58lck, Tyrosine-protein kinase Lck, p56lck, Protein YT16, Proto-oncogene Lck, YT16

Gene ID: 3932

UniProt: P06239

Pathways: TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Transition Metal Ion Homeostasis, Positive Regulation of Endopeptidase Activity, CXCR4-mediated Signaling Events, Thromboxane A2 Receptor Signaling