MHV NSP9 antibody

Catalog No. ABIN233751

Product Details

Target: MHV NSP9

Reactivity: Mouse Hepatitis Virus (MHV)

Host: Mouse

Clonality: Monoclonal

Conjugate: Un-conjugated

Application: Western Blotting (WB), Fluorescence Microscopy (FM)

Characteristics: Concentration Definition: by UV absorbance at 280 nm

Immunogen: This antibody was produced in mice by repeated immunizations with E.coli derived full-length MHV-A59 nsp9 protein. This protein is part of the viral replicase polyprotein.
Immunogentype:Native

Clone: 2C6-H1

Isotype: IgG

Application Details

Application Notes: This antibody has been tested for use in immunofluorescence microscopy and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 13 kDa in size corresponding to mature MHV-A59 nsp9 by western blotting in the appropriate cell lysate or extract. For immunofluorescence microscopy, Vero-E6 cells were grown on glass slides followed by infection with MHV-A59 strain and fixation with PBS/3%PFA.  After washing and permeabilization of the fixed cells, antibody incubation was performed in PBS/5%FCS for 30 min.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 2.1 mg/mL

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Target Details

Target: MHV NSP9

Target Type: Viral Protein

Background: The nonstructural protein 9 (nsp9) is one of the Mouse hepatitis virus replicase cleavage products, encoded by ORF1a. Nsp9 is an RNA-binding protein that is thought to be part of the viral replication complex, which is associated with intracellular membranes.
Synonyms: MHV-A59 nsp9 protein antibody

Gene ID: 1489749, 25121567

UniProt: P0C6V0