Feline Immunodeficiency Virus Matrix (MA) (FIV p15) (Internal Region) antibody

Catalog No. ABIN233823

Product Details

Target: Feline Immunodeficiency Virus Matrix (MA) (FIV p15)

Binding Specificity: Internal Region

Reactivity: Feline Immunodeficiency Virus (FIV)

Host: Rabbit

Clonality: Polyclonal

Conjugate: Un-conjugated

Application: ELISA, Western Blotting (WB)

Characteristics: Concentration Definition: by UV absorbance at 280 nm

Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids from an of FIV Matrix Protein p15.

Isotype: IgG

Application Details

Application Notes: This affinity purified antibody has been tested for use in ELISA, western blotting and immunocytochemistry.  Specific conditions for reactivity should be optimized by the end user.  Expect a band approximately 15 kDa in size corresponding to FIV Matrix Protein p15 by western blotting in the appropriate cell lysate or extract.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.22 mg/mL

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Target Details

Target: Feline Immunodeficiency Virus Matrix (MA) (FIV p15)

Target Type: Viral Protein

Background: This antibody is designed, produced, and is suitable for Cancer, Immunology and Nuclear Signaling research. Feline immunodeficiency virus (FIV) belongs to the lentivirus family.  This family is characterized by assembly of the viral capsid at either the plasma membrane or at the limiting membrane of late endosomes.  The capsid assembles from the viral Gag polyprotein.  Upon release of a budding virion, Gag precursor protein is cleaved by the viral protease into its mature products, namely Matrix Protein, Capsid and Nucleocapsid.  Matrix Protein, located at the N-terminus of the Gag polyprotein, is usually myristylated during protein translation, prior to the later events of virus assembly.  The myristate moiety is believed to be sequestered within the Matrix Protein during protein translation and later facilitates membrane binding upon exposure resulting from conformational changes.  Essential functions attributed to the Matrix Protein of lentiviruses include targeting newly synthesized Gag precursor proteins to the site of virus assembly by binding with cellular components such as phosphatidylinositides.  In the mature virus particle, the Matrix Protein provides internal structure to the virion within the capsid, but is not exposed at the surface of the particle.  Based on studies with HIV, it is postulated that FIV Matrix Protein may also serve additional functions, including nuclear localization of the viral core upon entry of the virus into a new host cell.
Synonyms: Gag polyprotein antibody, Core polyprotein antibody, FIV Matrix Protein p15 antibody

Gene ID: 1489988, 120815

UniProt: P16087