Collagen Type I antibody

Catalog No. ABIN238356

This anti-Collagen Type I antibody is a Rabbit Polyclonal antibody detecting Collagen Type I in IHC (fro), IF, IHC (p), RIA and EIA. Suitable for Chicken.

Quick Overview for Collagen Type I antibody (ABIN238356)

Target: Collagen Type I (COL1) (Collagen, Type I (COL1))

Reactivity: Chicken

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Collagen Type I antibody is un-conjugated

Application: Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Radioimmunoassay (RIA), Enzyme Immunoassay (EIA)

Product Details anti-Collagen Type I Antibody

Purification: Affinity chromatography

Immunogen: Purified collagen type I from chicken skin

Application Details

Application Notes: Immunflourescence 1: 40-1: 80. Immunohistochemistry on frozen sections. Immunohistochemistry on paraffin sections 1: 500 - 1: 1000 (please see also Protocols). Incubation time: 60 min at RT or 2-8 °C over night. Positive control: chicken skin. ELISA. RIA 1: 1000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Protocol: Pretreatment of tissue: After de-waxing the tissue slices are treated with 0. 2% hyaluronidase (app. 300 U/mg) in

Restrictions: For Research Use only

Handling

Reconstitution: Restore by adding 0.5 mL aqua destilled, for further dilution use appropriate antibody diluent.

Concentration: app. 1 mg/mL

Handling Advice: Avoid repeated freezing and thawing. Dilute only prior to immediate use

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody (undiluted) at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for Collagen Type I

Target: Collagen Type I (COL1) (Collagen, Type I (COL1))

Alternative Name: Collagen Type I

Background: Collagens consist in a family of highly specialized glycoproteins of which at least 16 genetically distinct types are known to date. The basal unit of a collagen molecule cosists in a tripel-helical structure formed by 3 alpha-chains. Predominant amino acids are glycine, proline and hydroxproline. Regularly also lysines and hydroxylysines occur, which are responsible for cross-linkage and glycosylation of the protein chains. Different composition of alpha-chains and different glycosylation contribute to the high variability of collagens in different tissues and organs. Type I collagen (95 kDa) is found in bone, cornea, skin and tendon.Synonyms: Alpha-1 type I collagen, Alpha-2 type I collagen, COL1A1, COL1A2

Gene ID: 9031

UniProt: P02457