Collagen Type I antibody

Catalog No. ABIN238359

This anti-Collagen Type I antibody is a Rabbit Polyclonal antibody detecting Collagen Type I in IHC (fro), IF, IHC (p), RIA and EIA. Suitable for Tunafish, Bonito, Salmon and Sole (Solea solea).

Quick Overview for Collagen Type I antibody (ABIN238359)

Target: Collagen Type I (COL1) (Collagen, Type I (COL1))

Reactivity: Tunafish, Bonito, Salmon, Sole (Solea solea)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Collagen Type I antibody is un-conjugated

Application: Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Radioimmunoassay (RIA), Enzyme Immunoassay (EIA)

Product Details anti-Collagen Type I Antibody

Specificity: This antibody is specific for Salmon Collagen type I. Salmon Collagen I OD > 1.0 in ELISA at 1/100 dilution. Tunafish Collagen I: < = 0.40 Goldfish Collagen I: < = 0.2* Human, Mouse and Rat Collagen I: < 0.2* *< 0.2 OD in Direct ELISA means negative reaction.

Purification: Affinity Chromatography

Immunogen: Purified Collagen type I from Salmon skin

Application Details

Application Notes: IF: 1: 40. IHC(P): 1: 200 - 1: 600, 60 min at RT or 2-8C over night. ELISA: 1: 100 - 1: 200 (OD > 0.500). RIA. Positive control: Salmon skin, liver or bone. Pretreatment: After de-waxing the tissue slices they are treated with 0.2 % hyaluronidase(app.300 U/mg) in TBS 15 min at 37C. There after non-specific binding is blocked byblocking serum or 3 % BSA in TBS. For peroxidase systems blocking with 1 % peroxidesolution in TBS for 30 min at RT is recommended.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Reconstitution: Restore with distilled water to initial volume, for further dilution use appropriate antibody diluent.

Concentration: app. 1 mg/mL

Buffer: PBS containing no BSA or preservatives

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in small aliquots) at -20 °C for longer.

Target Details for Collagen Type I

Target: Collagen Type I (COL1) (Collagen, Type I (COL1))

Alternative Name: Collagen Type I

Background: Collagens consist in a family of highly specialized glycoproteins of which at least 16 genetically distinct types are known to date. The basal unit of a collagen molecule cosists in a tripel-helical structure formed by 3 alpha-chains. Predominant amino acids are glycine, proline and hydroxproline. Regularly also lysines and hydroxylysines occur, which are responsible for cross-linkage and glycosylation of the protein chains. Different composition of alpha-chains and different glycosylation contribute to the high variability of collagens in different tissues and organs. Type I collagen (95 kDa) is found in bone, cornea, skin and tendon.Synonyms: Alpha-1 type I collagen, Alpha-2 type I collagen, COL1A1, COL1A2

Gene ID: 1277

NCBI Accession: NP_000079

UniProt: P02452