CHD7 antibody (Internal Region)

Catalog No. ABIN238550

The Goat Polyclonal anti-CHD7 antibody has been validated for ELISA and IHC. It is suitable to detect CHD7 in samples from Human.

Quick Overview for CHD7 antibody (Internal Region) (ABIN238550)

Target: CHD7 (Chromodomain Helicase DNA Binding Protein 7 (CHD7))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This CHD7 antibody is un-conjugated

Application: ELISA, Immunohistochemistry (IHC)

Grade: Verified

Product Details anti-CHD7 Antibody

Binding Specificity: Internal Region

Purpose: CHD7

Sequence: KDEIDEFANS PSE

Cross-Reactivity: Human, Mouse, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-KDEIDEFANSPSE, from the internal region of the protein sequence according to NP_060250.2.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: Paraffin embedded Human Brain (Cortex). Recommended concentration: 3.75 μg/mL.

Western Blot: Preliminary experiments gave bands at approx 250 kDa and 48 kDa in Rat Spinal Cord lysates after 1 μg/mL antibody staining. Please note that currently we cannot find an explanation in the literature for the bands we observe given the calculate

Peptide ELISA: antibody detection limit dilution 1:64000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for CHD7

Target: CHD7 (Chromodomain Helicase DNA Binding Protein 7 (CHD7))

Alternative Name: CHD7

Background: Chromodomain helicase DNA binding protein 7, FLJ20357, FLJ20361, IS3, KIAA1416, Scoliosis, idiopathic 3

Gene ID: 55636, 320790, 312974

NCBI Accession: NP_060250

Pathways: Sensory Perception of Sound, Chromatin Binding