GOT1 antibody (AA 22-35)

Catalog No. ABIN238592

The Goat Polyclonal anti-GOT1 antibody is suitable to detect GOT1 in samples from Human. It has been validated for WB and ELISA.

Quick Overview for GOT1 antibody (AA 22-35) (ABIN238592)

Target: GOT1 (Glutamic-Oxaloacetic Transaminase 1, Soluble (Aspartate Aminotransferase 1) (GOT1))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This GOT1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Grade: Verified

Product Details anti-GOT1 Antibody

Binding Specificity: AA 22-35

Purpose: GOT1 (aa 22-35)

Sequence: TADFREDPDP RKVN

Cross-Reactivity: Human, Mouse

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: C-TADFREDPDPRKVN

Isotype: IgG

Application Details

Application Notes:

Western Blot: Approx 40 kDa band observed in Human Liver lysates (calculated MW of 46.2 kDa according to NP_002070.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Berk et al, Proc Natl Acad Sci U S

Peptide ELISA: antibody detection limit dilution 1:16000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for GOT1

Target: GOT1 (Glutamic-Oxaloacetic Transaminase 1, Soluble (Aspartate Aminotransferase 1) (GOT1))

Alternative Name: GOT1

Background: GOT1, glutamic-oxaloacetic transaminase 1, soluble (aspartate aminotransferase 1), GIG18, aspartate aminotransferase 1, growth-inhibiting protein 18

Gene ID: 2805, 14718

NCBI Accession: NP_002070

Pathways: Hepatitis C, Monocarboxylic Acid Catabolic Process, Methionine Biosynthetic Process