Myosin VIIA antibody (MYO7A)

Details for Product anti-MYO7A Antibody No. ABIN2435036, Supplier: Log in to see
Antigen
  • 53D10S
  • BG:DS00929.11
  • CG7595
  • D
  • D2
  • DM7a
  • DMyoVIIa
  • Dm 35B
  • DmVIIA
  • DmVIIa
  • Dmel\\CG7595
  • Dro35B
  • ESTS:53D10S
  • Mhc35BC
  • MyoVIIA
  • anon-35Bb
  • br27
  • ck/MyoVIIA
  • l(1)35Ca
  • l(2)07130
  • l(2)35Ca
  • l(2)br27
  • l35Ca
  • myoVIIA
  • stc
  • stch
  • myo7a
  • MYO7A
  • NV17859
  • Hdb
  • Myo7
  • USH1B
  • nmf371
  • polka
  • sh-1
  • sh1
  • DFNA11
  • DFNB2
  • MYOVIIA
  • MYU7A
  • NSRD2
  • crinkled
  • myosin VIIAa
  • myosin VIIa
  • myosin VIIA
  • myosin 7A
  • myosin-VIIa
  • ck
  • myo7aa
  • LOAG_02192
  • MYO7A
  • myo7a
  • LOC663995
  • LOC100116446
  • Myo7a
Alternatives
anti-Cow (Bovine) Myosin VIIA antibody for Western Blotting
Reactivity
Human, Mouse (Murine), Rat (Rattus)
48
16
11
3
1
1
1
1
1
1
Host
Rabbit
44
5
Clonality
Polyclonal
Conjugate
This Myosin VIIA antibody is un-conjugated
2
2
2
1
1
1
1
1
1
1
1
1
1
1
Application
ELISA, Immunofluorescence (IF), Immunohistochemistry (IHC)
22
21
13
7
5
4
3
1
Options
Supplier
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Supplier Product No.
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'Independent Validation' Badge
Antigen MYO7A
Lot Number EC8591
Method validated Immunofluorescence
Positive Control

Organ of Corti tissue dissected from the cochlea of the inner ear, from a WT mouse, C57BL/6 strain, 10 weeks old.

Negative Control Primary and secondary only antibody controls
Notes

Passed. ABIN2435036 recognizes MYO7A with the expected expression pattern in murine organ of Corti tissue dissected from the cochlea of the inner ear.

Primary Antibody ABIN2435036
Secondary Antibody anti-rabbit AF546 conjugated antibody (Life Technologies, A11035, lot 1812311)
Protocol
  • Dissect organ of Corti from the mouse cochlea in the inner ear as described in Maison, Liberman, and Liberman (2016).
  • Cryoprotect and freeze/thaw to permeabilize the tissue:
    • Transfer cochlear pieces to a 5ml disposable cup with approximately 1ml of 30% sucrose in 100mM phosphate buffer (PBS) at RT.
    • Incubate tissue on a shaker for 15min at RT.
    • Wash tissue with 30% sucrose in PBS at RT.
    • Incubate tissue on a shaker for 15min at RT.
    • Place cup on dry ice until contents freeze completely.
    • Allow cup to thaw at RT.
    • Pipet out the sucrose solution and wash tissue 3x for 15min with PBS containing 0.1% triton X-100 on a shaker at RT.
  • Block tissue with blocking solution (5% goat serum containing 0.3% Triton-X) on a shaker for 30-60min at RT.
  • Pipet out PBS + detergent and add blocking solution.
  • Cut cap off a 1.5ml microcentrifuge tube and transfer tissue pieces in the blocking solution to the flipped upside-down cap.
  • Incubate tissue in the flipped upside-down caps with 100µl primary
    • rabbit anti-MYO7A antibody (antibodies-online, ABIN2435036, lot EC8591) diluted 1:100 in blocking solution ON at RT on an agitator.
    • chicken anti-parvalbumin (Synaptic Systems, 195006) diluted 1:400 in blocking solution ON at RT on an agitator.
  • Fasten tubes onto the caps and and protect them from the light.
  • Pipet out the primary antibody solution.
  • Rinse tissue 3x for a total of 10min with PBS containing 0.1% Triton X-100.
  • Incubate tissue in the flipped upside-down caps with 100µl secondary
    • goat anti-rabbit AF546 conjugated antibody (Life Technologies, A11035, lot 1812311) diluted 1:300 in blocking solution for 1h at RT away from the light.
    • goat anti-chicken AF488 conjugated antibody (Life Technologies, A11039, lot 1812246) diluted 1:300 in blocking solution for 1h at RT away from the light.
  • Rinse tissue 3x for a total of 10min with PBS.
  • Transfer tissue pieces onto a slide with stereocilia facing up.
  • Add mounting Fluoromount-G with DAPI mounting medium (ThermoFisher Scientific, 00-4959-52, lot B2215-N915) then coverslip.
  • Image acquisition on a Zeiss Axiovert epifluorescence with with a 63x objective, using a Zeiss ApoTome for optical sectioning.
Experimental Notes
  • The observed signal on outer hair cells in the organ of Corti for ABIN2435036 appears as expected, compared to numerous other publications. For example, Figure 4 in He et al., and Figure 1 in Kaur et al., and Figure 4 in Li et al..

  • ABIN2435036 worked well enough to detect the hair cells at 1:100 dilution. A more concentrated dilution would likely improve the signal to noise ratio.

  • No signal was observed with either negative control.

Validation Images
Immunofluorescence validation image for anti-Myosin VIIA (MYO7A) antibody (ABIN2435036) In this photograph of the 3 rows of cochlear outer hair cells taken with 63x objectiv...
Immunogen Synthetic peptide of human MYO7A
Isotype IgG
Purification Antigen affinity purification
Plasmids, Primers & others Plasmids, Primers & others Myosin VIIA products on genomics-online (e.g. as negative or positive controls)
Antigen
Alternative Name MYO7A (MYO7A Antibody Abstract)
Background This gene is a member of the myosin gene family. Myosins are mechanochemical proteins characterized by the presence of a motor domain, an actin-binding domain, a neck domain that interacts with other proteins, and a tail domain that serves as an anchor. This gene encodes an unconventional myosin with a very short tail. Defects in this gene are associated with the mouse shaker-1 phenotype and the human Usher syndrome 1B which are characterized by deafness, reduced vestibular function, and (in human) retinal degeneration. Alternative splicing results in multiple transcript variants.
Synonyms: DFNB2, MYU7A, NSRD2, USH1B, DFNA11, MYOVIIA
Pathways Sensory Perception of Sound
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only
Format Liquid
Handling Advice Avoid freeze / thaw cycles.
Storage -20 °C/-80 °C
Storage Comment Store at -20°C (regular) and -80°C (long term).
Supplier Images
Immunohistochemistry (IHC) image for anti-Myosin VIIA (MYO7A) antibody (ABIN2435036) Immunohistochemistry of Human brain using MYO7A Polyclonal Antibody at dilution of 1:30
Immunohistochemistry (IHC) image for anti-Myosin VIIA (MYO7A) antibody (ABIN2435036) anti-Myosin VIIA (MYO7A) antibody (Image 2)
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