GFP antibody (Alkaline Phosphatase (AP))

Catalog No. ABIN243771

This anti-GFP antibody is a Mouse Monoclonal antibody detecting GFP in WB, ELISA and IHC. Suitable for Aequorea victoria.

Quick Overview for GFP antibody (Alkaline Phosphatase (AP)) (ABIN243771)

Target: GFP (Green Fluorescent Protein (GFP))

Reactivity: Aequorea victoria

Host: Mouse

Clonality: Monoclonal

Conjugate: This GFP antibody is conjugated to Alkaline Phosphatase (AP)

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Product Details anti-GFP Antibody (Alkaline Phosphatase (AP))

Specificity: Recognizes wild type, recombinant and enhanced forms of GFP. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-mouse serum and purified and partially purified Green Fluorescent Protein (Aequorea victoria).

Purification: Protein A purified

Immunogen: GST- Green Fluorescent Protein (GFP) fusion protein corresponding to the full length amino acid sequence (246aa) derived from the jellyfish Aequorea victoria.

Type of Immunogen: Fusion protein

Isotype: IgG1 kappa

Application Details

Application Notes: Approved: ELISA (1:1000 - 1:5000), IHC, WB

Usage: Suitable for use in ELISA (sandwich or capture), Western Blot and Immunohistochemistry. in a standard capture ELISA using pNPP p-nitrophenyl phosphate as a substrate for 30 minutes at RT. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

Comment:

Target Species of Antibody: Aequorea victoria

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: 0.05 M Tris chloride, 0.15 M sodium chloride, 0.001 M magnesium chloride, 0.0001 M zinc chloride, 50 % glycerol, 10 mg/mL BSA, (IgG, protease free), 0.01 % sodium azide,  pH 8.0.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: avoid freezing

Storage: 4 °C

Storage Comment: 4°C, avoid freezing.

Target Details for GFP

Target: GFP (Green Fluorescent Protein (GFP))

Alternative Name: GFP

Background: Name/Gene ID: GFP

Synonyms: GFP

UniProt: P42212