Presenilin 1 antibody (AA 21-34)

Catalog No. ABIN2443726

This Mouse Monoclonal antibody specifically detects Presenilin 1 in WB, ELISA, IHC and IF. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for Presenilin 1 antibody (AA 21-34) (ABIN2443726)

Target: Presenilin 1 (PSEN1)

Reactivity: Human, Mouse, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This Presenilin 1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF)

Clone: APS11

Product Details anti-Presenilin 1 Antibody

Binding Specificity: AA 21-34

Purpose: Presenilin 1 (C term) Mouse Monoclonal Antibody (APS11)

Specificity: The antibody recognizes the C-terminal region of the Presenilin 1 with no cross-reactivity observed with Presenilin 2

Immunogen: aa 21-34

Isotype: IgG1

Application Details

Application Notes: ELISA 1:200,WB 1:100,IF 1:1300,IHC 1:1300

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: If reconstituted with deionized water in 250μL: ELISA 1:200, WB 1:100, IF 1:1300, IHC 1:1300. Optimal dilution has to be determined by the user.

Buffer: Lyophilized protein free tissue culture supernatant

Preservative: Without preservative

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Lyophilized antibodies can be kept at 4°C for up to 3 months and should be kept at -20°C for long-term storage (2 years). To avoid freeze-thaw cycles, reconstituted antibodies should be aliquoted before freezing for long-term (1 year) storage (-80°C) or kept at 4°C for short-term usage (2 months). For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made with the assay buffer. After the maximum long-term storage period (2 years lyophilized or 1 year reconstituted) antibodies should be tested in your assay with a standard sample to verify if you have noticed any decrease in their efficacy. To limit antibody loss or degradation, BSA (final concentration 1%) and sodium azide (final concentration 0.02%) can be added to the suggested first dilution. It is important to first verify if those preservatives are compatible with your assay.

Expiry Date: 24 months

Target Details for Presenilin 1

Target: Presenilin 1 (PSEN1)

Alternative Name: Presenilin 1

Background:

PS-1, Protein S182, PSEN1

Presenilin is an integral membrane protein ubiquitously expressed in the brain, it is mainly expressed in neurons where it is localized in the endoplasmic reticulum. Mutations in the presenilin proteins (PSEN1, PSEN2) are linked to an inherited form of Alzheimer's disease in some patients by increasing the production of the longer form of amyloid-beta. Presenilins are thought to affect gamma-secretase by regulating amyloid precursor protein (APP) processing and cleavage of the Notch receptor.

UniProt: P49768

Pathways: Notch Signaling, EGFR Signaling Pathway, Synaptic Vesicle Exocytosis, Dicarboxylic Acid Transport