ARF1 antibody

Catalog No. ABIN2443832

The Rabbit Polyclonal anti-ARF1 antibody is suitable to detect ARF1 in samples from Human and Cow. It has been validated for WB and IHC.

Quick Overview for ARF1 antibody (ABIN2443832)

Target: ARF1 (ADP-Ribosylation Factor 1 (ARF1))

Reactivity: Human, Cow

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ARF1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-ARF1 Antibody

Purpose: ADP ribosylation factor 1 (Arf1) Rabbit Polyclonal Antibody

Specificity: A specific antibody against the bovine ADP ribosylation factor 1 protein (Arf1).

Application Details

Application Notes: WB 1:2000-1:3000

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: If reconstituted with deionized water in 50 μL: WB 1:2000-1:3000, IHC: 1:100-1:250. Optimal dilution has to be determined by the user.

Buffer: Lyophilized serum

Preservative: Without preservative

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Lyophilized antibodies can be kept at 4°C for up to 3 months and should be kept at -20°C for long-term storage (2 years). To avoid freeze-thaw cycles, reconstituted antibodies should be aliquoted before freezing for long-term (1 year) storage (-80°C) or kept at 4°C for short-term usage (2 months). For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made with the assay buffer. After the maximum long-term storage period (2 years lyophilized or 1 year reconstituted) antibodies should be tested in your assay with a standard sample to verify if you have noticed any decrease in their efficacy. To limit antibody loss or degradation, BSA (final concentration 1%) and sodium azide (final concentration 0.02%) can be added to the suggested first dilution. It is important to first verify if those preservatives are compatible with your assay.

Expiry Date: 24 months

Target Details for ARF1

Target: ARF1 (ADP-Ribosylation Factor 1 (ARF1))

Alternative Name: ADP ribosylation factor 1 (Arf1)

Background: ADP ribosylation factor 1 (Arf1) is a GTP-binding protein that functions as an allosteric activator of the cholera toxin catalytic subunit, an ADP-ribosyltransferase. It is involved in protein trafficking among different compartments, such as in the Golgi complex.

UniProt: P84077

Pathways: Transition Metal Ion Homeostasis, Inositol Metabolic Process