CRB3 antibody (full length)

Catalog No. ABIN2443902

The Rat Monoclonal anti-CRB3 antibody (Clone 14F9) (ABIN2443902) specifically detects CRB3 in WB, ELISA, IF and IHC. The antibody is reactive with Human and Mouse samples.

Quick Overview for CRB3 antibody (full length) (ABIN2443902)

Target: CRB3 (Crumbs Homolog 3 (CRB3))

Reactivity: Human, Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This CRB3 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunohistochemistry (IHC)

Clone: 14F9

Product Details anti-CRB3 Antibody

Binding Specificity: full length

Purpose: CRB3 Rat Monoclonal Antibody (14F9)

Specificity: The antibody recognizes the full length CRB3 protein but not the extra cellular domain of CRB3. Recognize the overexpressed human form and the endogenous form in mouse.

Purification: Protein G purified

Immunogen: Full length protein

Isotype: IgG2c

Application Details

Application Notes: WB 1:1000,IHC 1:100,IF 1:100

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: If reconstituted with deionized water in 100 μL: WB 1:1000, IHC 1:100, IF 1:100. Optimal dilution has to be determined by the user.

Buffer: Lyophilized protein G purified in PBS pH 7.4

Preservative: Without preservative

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Lyophilized antibodies can be kept at 4°C for up to 3 months and should be kept at -20°C for long-term storage (2 years). To avoid freeze-thaw cycles, reconstituted antibodies should be aliquoted before freezing for long-term (1 year) storage (-80°C) or kept at 4°C for short-term usage (2 months). For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made with the assay buffer. After the maximum long-term storage period (2 years lyophilized or 1 year reconstituted) antibodies should be tested in your assay with a standard sample to verify if you have noticed any decrease in their efficacy. To limit antibody loss or degradation, BSA (final concentration 1%) and sodium azide (final concentration 0.02%) can be added to the suggested first dilution. It is important to first verify if those preservatives are compatible with your assay.

Expiry Date: 24 months

Target Details for CRB3

Target: CRB3 (Crumbs Homolog 3 (CRB3))

Alternative Name: CRB3

Background:

Crumbs homologue 3

CRB3 (Crumbs homologue 3) is an apical transmembrane protein member of the CRB protein family reported to be associated with tight junctions at the apical surface of epithelial cells and to play a role in apical membrane morphogenesis and tight junction regulation. The formation and maintenance of tight junctions is essential for the development of epithelial cell polarity. Two CRB3 isoforms have been described and CRB3 is homologous to the Crumb protein found in Drosophila. CRB3 contains a putative signal peptide in its first 20 amino acids, a putative extracellular site, and a transmembrane domain. The intracellular domain contains a PDZ-binding motif which is involved in the interactions of CRB3 with PARD6A and MPP5. Loss of CRB3 expression in immortal baby mouse kidney (iBMK) cells was reported to render these cells tumorigenic, suggesting an important role for CRB3 function in maintaining epithelial junction formation and apicobasal polarity required for contact-inhibited growth, and to suppress invasion and metastasis.

UniProt: Q9BUF7

Pathways: Notch Signaling, Cell-Cell Junction Organization