EpCAM antibody

Catalog No. ABIN2443916

The Mouse Monoclonal anti-EpCAM antibody is suitable to detect EpCAM in samples from Human and Mouse. It has been validated for WB, IHC and ELISA.

Quick Overview for EpCAM antibody (ABIN2443916)

Target: EpCAM (EPCAM) (Epithelial Cell Adhesion Molecule (EPCAM))

Reactivity: Human, Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This EpCAM antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Clone: 4A7

Product Details anti-EpCAM Antibody

Purpose: Epcam ICD Mouse Monoclonal Antibody (4A7)

Specificity: The antibody recognizes the intracellular domain of human EpCAM.

Purification: Protein G purified

Isotype: IgG1

Application Details

Application Notes: WB 1:1000

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: If reconstituted with deionized water in 100 μL: WB 1:1000. Optimal dilution has to be determined by the user.

Buffer: Lyophilized protein G purified in PBS pH 7.4

Preservative: Without preservative

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Lyophilized antibodies can be kept at 4°C for up to 3 months and should be kept at -20°C for long-term storage (2 years). To avoid freeze-thaw cycles, reconstituted antibodies should be aliquoted before freezing for long-term (1 year) storage (-80°C) or kept at 4°C for short-term usage (2 months). For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made with the assay buffer. After the maximum long-term storage period (2 years lyophilized or 1 year reconstituted) antibodies should be tested in your assay with a standard sample to verify if you have noticed any decrease in their efficacy. To limit antibody loss or degradation, BSA (final concentration 1%) and sodium azide (final concentration 0.02%) can be added to the suggested first dilution. It is important to first verify if those preservatives are compatible with your assay.

Expiry Date: 24 months

Target Details for EpCAM

Target: EpCAM (EPCAM) (Epithelial Cell Adhesion Molecule (EPCAM))

Background:

Epithelial Cell Adhesion Molecule Intracellular Domain

Epithelial cell adhesion molecule (EpCAM) is a 40- kDa epithelial transmembrane glycoprotein with an ectodomain, one transmembrane domain, and a cytoplasmic domain of 26 residues. EpCAM is frequently overexpressed in human malignancies, normal stem and progenitor cells, and at lower levels in normal epithelia. In vivo and in vitro studies have shown its intracellular domain (ICD) fragment translocates to nucleus after being cleaved from the EpCAM ectodomain and associates with other molecules to bind DNA resulting in cell proliferation and tumor formation.

UniProt: P16422