CBX3 antibody (C-Term)

Catalog No. ABIN2452035

Cited in 3+ publications. The Rabbit Polyclonal anti-CBX3 antibody (ABIN2452035) specifically detects CBX3 in WB, IF and ChIP-seq. The antibody is reactive with Human and Hamster samples.

Quick Overview for CBX3 antibody (C-Term) (ABIN2452035)

Target: CBX3 (Chromobox Homolog 3 (CBX3))

Reactivity: Human, Hamster

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CBX3 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), ChIP DNA-Sequencing (ChIP-seq)

Product Details anti-CBX3 Antibody

Binding Specificity: C-Term

Cross-Reactivity (Details): Expected to react with chicken, Xenopus, Drosophila, and zebra fish orthologs due to the sequence identity of the immunogen.

Characteristics: The product is prepared by immunizing rabbit with the synthetic peptide WHSCPEDEAQ-C corresponding to the C-terminal sequence of human HP1γ and purified by affinity purification with the peptide. The antiserum preparation has been directed by Prof. T. Haraguchi.

Purification: Affinity purified

Sterility: Sterile filtered

Isotype: IgG

Application Details

Application Notes: 1) Western blotting: 1/2,000~1/10,000
2) Immunofluorescence staining
3) Chromatin immunoprecipitation (ChIP)

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.75 mg/mL

Buffer: 0.12 M sodium phosphate buffer ( pH 7.4), 50 % glycerol

Preservative: Azide free

Storage: -20 °C/-80 °C

Storage Comment: -20 C (For long term storage: -70 C)

References for anti-CBX3 antibody (ABIN2452035)

Lomberk, Wallrath, Urrutia: "The Heterochromatin Protein 1 family." in: Genome biology, Vol. 7, Issue 7, pp. 228, (2007) (PubMed).

Wang, Koyama, Nishida, Haraguchi, Reith, Tsukamoto: "The assembly and maintenance of heterochromatin initiated by transgene repeats are independent of the RNA interference pathway in mammalian cells." in: Molecular and cellular biology, Vol. 26, Issue 11, pp. 4028-40, (2006) (PubMed).

Kametaka, Takagi, Hayakawa, Haraguchi, Hiraoka, Yoneda: "Interaction of the chromatin compaction-inducing domain (LR domain) of Ki-67 antigen with HP1 proteins." in: Genes to cells : devoted to molecular & cellular mechanisms, Vol. 7, Issue 12, pp. 1231-42, (2002) (PubMed).

Target Details for CBX3

Target: CBX3 (Chromobox Homolog 3 (CBX3))

Alternative Name: HP1gamma/CBX3

Background: Heterochromatin protein 1 (HP1) is a major component of heterochromatin which plays a role in assembly of various proteins on chromatin and gene silencing. The HP1 family is evolutionally conserved, with members in fungi, plants and animals but not prokaryotes, and there are multiple members within the same species. The HP1 family proteins are encoded by a class of genes known as the chromobox (CBX) genes. In humans, HP1γ is encoded by the Chromobox homolog 3 (CBX3) gene. HP1γ has been observed to interact directly or indirectly with several non-histone proteins with a wide variety of functions.

UniProt: Q13185