NFYA antibody

Catalog No. ABIN2460199

Product Details anti-NFYA Antibody

Target: NFYA (Nuclear Transcription Factor Y, alpha (NFYA))

Reactivity: Human, Mouse, Rat, Dog

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NFYA antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Purification: Antibody is supplied as total IgG.

Immunogen: Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human NFYA.

Application Details

Application Notes: NFYA antibody can be used for detection of NFYA by ELISA at 1:312500. NFYA antibody can be used for detection of NFYA by western blot at 5.0-8.0 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 200 ?L of distilled water. Final antibody concentration is 1 mg/mL.

Concentration: 1 mg/mL

Buffer: Antibody is lyophilized in PBS buffer with 2 % sucrose.

Handling Advice: As with any antibody avoid repeat freeze-thaw cycles.

Storage: 4 °C/-20 °C

Storage Comment: For short periods of storage (days) store at 4 °C. For longer periods of storage, store NFYA antibody at -20 °C.

Target Details for NFYA

Target: NFYA (Nuclear Transcription Factor Y, alpha (NFYA))

Alternative Name: NFYA (NFYA Products)

Synonyms: wu:fb16e03 antibody, ik:tdsubc_2b6 antibody, xx:tdsubc_2b6 antibody, NFYA antibody, CBF-A antibody, CBF-B antibody, HAP2 antibody, NF-YA antibody, AA407810 antibody, Cbf-b antibody, SEZ-10 antibody, SEZ10 antibody, nuclear transcription factor Y, alpha antibody, nuclear transcription factor Y subunit alpha antibody, nuclear transcription factor-Y alpha antibody, nfya antibody, NFYA antibody, Nfya antibody

Background: NFYA is one subunit of a trimeric complex, forming a highly conserved transcription factor that binds to CCAAT motifs in the promoter regions in a variety of genes. Subunit A associates with a tight dimer composed of the B and C subunits, resulting in a trimer that binds to DNA with high specificity and affinity. The sequence specific interactions of the complex are made by the A subunit, suggesting a role as the regulatory subunit. In addition, there is evidence of post-transcriptional regulation in this gene product, either by protein degradation or control of translation. Further regulation is represented by alternative splicing in the glutamine-rich activation domain, with clear tissue-specific preferences for the two isoforms.

Molecular Weight: 37 kDa, 34 kDa

Gene ID: 4800

NCBI Accession: NP_002496

UniProt: P23511

Pathways: Regulation of Lipid Metabolism by PPARalpha