POLR2B antibody
-
- Target See all POLR2B Antibodies
- POLR2B (Polymerase (RNA) II (DNA Directed) Polypeptide B, 140kDa (POLR2B))
-
Reactivity
- Human, Mouse, Rat, Dog
-
Host
- Rabbit
-
Clonality
- Polyclonal
-
Conjugate
- This POLR2B antibody is un-conjugated
-
Application
- Western Blotting (WB), ELISA
- Purification
- Antibody is purified by peptide affinity chromatography method.
- Immunogen
- Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human POLR2B.
- Top Product
- Discover our top product POLR2B Primary Antibody
-
-
- Application Notes
- POLR2B antibody can be used for detection of POLR2B by ELISA at 1:1562500. POLR2B antibody can be used for detection of POLR2B by western blot at 0.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
- Restrictions
- For Research Use only
-
- Format
- Lyophilized
- Reconstitution
- Add 50 ?L of distilled water. Final antibody concentration is 1 mg/mL.
- Concentration
- 1 mg/mL
- Buffer
- Antibody is lyophilized in PBS buffer with 2 % sucrose.
- Handling Advice
- As with any antibody avoid repeat freeze-thaw cycles.
- Storage
- 4 °C/-20 °C
- Storage Comment
- For short periods of storage (days) store at 4 °C. For longer periods of storage, store POLR2B antibody at -20 °C.
-
- Target
- POLR2B (Polymerase (RNA) II (DNA Directed) Polypeptide B, 140kDa (POLR2B))
- Alternative Name
- POLR2B (POLR2B Products)
- Synonyms
- Pol2rb antibody, Rpb140 antibody, Rpb2 antibody, POL2RB antibody, RPB2 antibody, hRPB140 antibody, hsRPB2 antibody, RNA polymerase II subunit B antibody, polymerase (RNA) II (DNA directed) polypeptide B, 140kDa antibody, DNA-directed RNA polymerase II subunit RPB2 antibody, polymerase (RNA) II (DNA directed) polypeptide B antibody, polymerase (RNA) II (DNA directed) polypeptide B, 140kDa L homeolog antibody, POLR2B antibody, polr2b antibody, LOC100640211 antibody, Polr2b antibody, polr2b.L antibody, rpb-2 antibody
- Background
- POLR2B encodes the second largest subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. This subunit, in combination with at least two other polymerase subunits, forms a structure within the polymerase that maintains contact in the active site of the enzyme between the DNA template and the newly synthesized RNA.
- Molecular Weight
- 134 kDa
- Gene ID
- 5431
- NCBI Accession
- NP_000929
- UniProt
- P30876
- Pathways
- Regulatory RNA Pathways, DNA Damage Repair
-