SMAD6 antibody

Catalog No. ABIN2460536

The Rabbit Polyclonal anti-SMAD6 antibody has been validated for WB, ELISA and IHC. It is suitable to detect SMAD6 in samples from Human, Mouse, Rat and Dog.

Quick Overview for SMAD6 antibody (ABIN2460536)

Target: SMAD6 (SMAD, Mothers Against DPP Homolog 6 (SMAD6))

Reactivity: Human, Mouse, Rat, Dog

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SMAD6 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Product Details anti-SMAD6 Antibody

Purification: Antibody is supplied as total IgG.

Immunogen: Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human SMAD6.

Application Details

Application Notes: SMAD6 antibody can be used for detection of SMAD6 by ELISA at 1:62500. SMAD6 antibody can be used for detection of SMAD6 by western blot at 0.5-1.0 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 200 ?L of distilled water. Final antibody concentration is 1 mg/mL.

Concentration: 1 mg/mL

Buffer: Antibody is lyophilized in PBS buffer with 2 % sucrose.

Handling Advice: As with any antibody avoid repeat freeze-thaw cycles.

Storage: 4 °C/-20 °C

Storage Comment: For short periods of storage (days) store at 4 °C. For longer periods of storage, store SMAD6 antibody at -20 °C.

Target Details for SMAD6

Target: SMAD6 (SMAD, Mothers Against DPP Homolog 6 (SMAD6))

Alternative Name: SMAD6

Background: SMAD6 (mothers against decapentaplegic homolog 6) is an antagonist of signaling by TGF-beta (transforming growth factor) type 1 receptor superfamily members, has been shown to inhibit selectively BMP (bone morphogenetic proteins) signaling by competing with the co-SMAD SMAD4 for receptor-activated SMAD1. SMAD6 is an inhibitory SMAD (I-SMAD) or antagonistic SMAD.

Molecular Weight: 53 kDa

Gene ID: 4091

NCBI Accession: NP_005576

UniProt: O43541

Pathways: Chromatin Binding